1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps16, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf1, ycf2, ycf2_copy2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome rpl23 Small subunit of ribosome None DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [12785:12928](-) [11667:12074](-) clpP (3/3) [73903:73973](-) [72818:73109](-) [71956:72210](-) ndhA (2/2) [122626:123182](-) [120678:121227](-) ndhB (2/2) [97219:97995](-) [95798:96547](-) ndhB_copy2 (2/2) [142893:143669](+) [144341:145090](+) petB (2/2) [76926:76931](+) [77690:78331](+) petD (2/2) [78534:78541](+) [79247:79721](+) rpl16 (2/2) [84604:84612](-) [83156:83590](-) rpl2 (1/2) [86292:87116](-) rpoC1 (2/2) [23410:23841](-) [20981:22591](-) rps12 (3/3) [71680:71793](-) [141301:141532](+) [142069:142094](+) rps16 (2/2) [5589:5628](-) [4548:4768](-) ycf3 (3/3) [45669:45794](-) [44722:44949](-) [43787:43939](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbL ACG rps19 GTG ndhD GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 117 genes (107 unique genes). Including: 80 protein-coding genes (77 are unique) 37 tRNA genes (30 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 16S ribosomal RNA [137907:139397](-) 23S ribosomal RNA [132700:135508](-) 4.5S ribosomal RNA [132496:132599](-) 5S ribosomal RNA [132157:132278](-) 80 protein-coding genes (77 are unique) Genes Length Location accD 1572 [58764:60335](+) atpA 1515 [10089:11603](-) atpB 1473 [54548:56020](-) atpE 423 [54103:54525](-) atpF 552 [12785:12928](-) [11667:12074](-) atpH 246 [13386:13631](-) atpI 744 [14708:15451](-) ccsA 954 [115343:116296](+) cemA 690 [62880:63569](+) clpP 618 [73903:73973](-) [72818:73109](-) [71956:72210](-) matK 1509 [1595:3103](-) ndhA 1107 [122626:123182](-) [120678:121227](-) ndhB 1527 [97219:97995](-) [95798:96547](-) ndhB_copy2 1527 [142893:143669](+) [144341:145090](+) ndhC 363 [51717:52079](-) ndhD 1527 [116568:118094](-) ndhE 306 [118703:119008](-) ndhF 2133 [111171:113303](-) ndhG 531 [119242:119772](-) ndhH 1182 [123184:124365](-) ndhI 504 [120093:120596](-) ndhJ 498 [50359:50856](-) ndhK 708 [50944:51651](-) petA 963 [63797:64759](+) petB 648 [76926:76931](+) [77690:78331](+) petD 483 [78534:78541](+) [79247:79721](+) petG 114 [67881:67994](+) petL 96 [67555:67650](+) petN 90 [29288:29377](+) psaA 2253 [40765:43017](-) psaB 2205 [38535:40739](-) psaC 246 [118192:118437](-) psaI 102 [61096:61197](+) psaJ 135 [68926:69060](+) psbA 1062 [1:1062](-) psbB 1527 [74488:76014](+) psbC 1422 [34998:36419](+) psbD 1062 [33989:35050](+) psbE 252 [66151:66402](-) psbF 120 [66019:66138](-) psbH 222 [76575:76796](+) psbI 111 [7471:7581](+) psbJ 123 [65623:65745](-) psbK 180 [6948:7127](+) psbL 117 [65880:65996](-) psbM 105 [30664:30768](-) psbN 132 [76331:76462](-) psbT 108 [76163:76270](+) psbZ 189 [37108:37296](+) rbcL 1443 [56441:57883](+) rpl14 369 [82684:83052](-) rpl16 444 [84604:84612](-) [83156:83590](-) rpl2 825 [86292:87116](-) rpl20 351 [70512:70862](-) rpl22 480 [85417:85896](-) rpl32 174 [113984:114157](+) rpl33 201 [69519:69719](+) rpl36 114 [81503:81616](-) rpoA 1014 [79905:80918](-) rpoB 3189 [23868:27056](-) rpoC1 2043 [23410:23841](-) [20981:22591](-) rpoC2 4149 [16658:20806](-) rps11 417 [80995:81411](-) rps12 372 [71680:71793](-) [141301:141532](+) [142069:142094](+) rps14 303 [38107:38409](-) rps15 273 [124488:124760](-) rps16 261 [5589:5628](-) [4548:4768](-) rps18 345 [69909:70253](+) rps19 282 [85962:86243](-) rps2 711 [15683:16393](-) rps3 657 [84776:85432](-) rps4 612 [46951:47562](-) rps7 468 [98273:98740](-) rps7_copy2 468 [142148:142615](+) rps8 411 [82101:82511](-) ycf1 5838 [125127:130964](-) ycf2 6606 [87886:94491](+) ycf2_copy2 6606 [146397:153002](-) ycf3 507 [45669:45794](-) [44722:44949](-) [43787:43939](-) ycf4 555 [61633:62187](+) 37 tRNA genes (30 are unique) Genes Location trnK-UUU [3835:3871](-) [1280:1315](-) trnQ-UUG [6506:6577](-) trnS-GCU [7721:7807](-) trnG-UCC [8421:8443](+) [9350:9397](+) trnR-UCU [9902:9973](+) trnC-GCA [28286:28357](+) trnD-GUC [31444:31517](-) trnY-GUA [31624:31707](-) trnE-UUC [31763:31835](-) trnT-GGU [32598:32669](+) trnS-UGA [36666:36755](-) trnG-GCC [37571:37640](+) trnfM-CAU [37892:37965](-) trnS-GGA [46547:46633](+) trnT-UGU [48015:48087](-) trnL-UAA [48874:48909](+) [49251:49299](+) trnF-GAA [49671:49743](+) trnV-UAC [53618:53655](-) [52998:53034](-) trnM-CAU [53836:53908](+) trnW-CCA [68119:68192](-) trnP-UGG [68368:68441](-) trnI-CAU [87658:87731](-) trnL-CAA [95196:95276](-) trnV-GAC [101197:101268](+) trnI-GAU [103279:103320](+) [104245:104279](+) trnA-UGC [104343:104380](+) [105192:105226](+) trnR-ACG [108970:109043](+) trnN-GUU [109597:109668](-) trnL-UAG [115138:115217](+) trnN-GUU_copy2 [131220:131291](+) trnR-ACG_copy2 [131845:131918](-) trnA-UGC_copy2 [136508:136545](-) [135662:135696](-) trnI-GAU_copy2 [137568:137609](-) [136609:136643](-) trnV-GAC_copy2 [139620:139691](-) trnL-CAA_copy2 [145612:145692](+) trnI-CAU_copy2 [153157:153230](+) trnH-GUG [153465:153539](-) 0 rRNA genes (0 are unique) Genes Location This genome has 20 genes with intron(s). Details are as follows: 17 gene(s) have 1 intron(s) Genes Exons rps16 [5589:5628](-) [4548:4768](-) atpF [12785:12928](-) [11667:12074](-) rpoC1 [23410:23841](-) [20981:22591](-) petB [76926:76931](+) [77690:78331](+) petD [78534:78541](+) [79247:79721](+) rpl16 [84604:84612](-) [83156:83590](-) ndhB [97219:97995](-) [95798:96547](-) ndhA [122626:123182](-) [120678:121227](-) ndhB_copy2 [142893:143669](+) [144341:145090](+) trnK-UUU [3835:3871](-) [1280:1315](-) trnG-UCC [8421:8443](+) [9350:9397](+) trnL-UAA [48874:48909](+) [49251:49299](+) trnV-UAC [53618:53655](-) [52998:53034](-) trnI-GAU [103279:103320](+) [104245:104279](+) trnA-UGC [104343:104380](+) [105192:105226](+) trnA-UGC_copy2 [136508:136545](-) [135662:135696](-) trnI-GAU_copy2 [137568:137609](-) [136609:136643](-) 3 gene(s) have 2 intron(s) Genes Exons ycf3 [45669:45794](-) [44722:44949](-) [43787:43939](-) rps12 [71680:71793](-) [141301:141532](+) [142069:142094](+) clpP [73903:73973](-) [72818:73109](-) [71956:72210](-)