1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpH Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I psaI Subunits of photosystem II psbM Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps14, rps14_copy2, rps15, rps15_copy2, rps16, rps18, rps19, rps19_copy2, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf15, ycf15_copy2, ycf1_copy2, ycf2, ycf2_copy2 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbN, psbT, psbZ, ycf3 Large subunit of ribosome None Small subunit of ribosome rps12 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf4 Other genes None 2. Multicopy genes with different lengths: Genes Length Location rps14 303 [23804:24107](-) rps14_copy2 120 [37703:37823](-) 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 clpP (3/3) [44704:44774](-) [43671:43964](-) [42804:43029](-) rpl16 (2/2) [49176:49184](-) [47883:48284](-) rpl2 (2/2) [52012:52402](-) [50911:51341](-) rpl2_copy2 (2/2) [109806:110196](+) [110867:111297](+) rps16 (2/2) [6001:6040](-) [4930:5123](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon None None 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 80 genes (58 unique genes). Including: 39 protein-coding genes (30 are unique) 35 tRNA genes (25 are unique) 6 rRNA genes (3 are unique) Details are as follows: 39 protein-coding genes (30 are unique) Genes Length Location accD 1692 [34896:36587](+) atpH 249 [12724:12972](-) clpP 591 [44704:44774](-) [43671:43964](-) [42804:43029](-) infA 234 [46484:46717](-) matK 1536 [1759:3294](-) psaI 51 [37229:37279](+) psbM 96 [18729:18824](-) rpl14 369 [47389:47757](-) rpl16 411 [49176:49184](-) [47883:48284](-) rpl2 822 [52012:52402](-) [50911:51341](-) rpl20 387 [41346:41732](-) rpl22 474 [49993:50466](-) rpl23 210 [71350:71559](-) rpl23_copy2 210 [90649:90858](+) rpl2_copy2 822 [109806:110196](+) [110867:111297](+) rpl32 147 [81047:81193](-) rpl33 201 [40515:40715](+) rpl36 114 [46271:46384](-) rps11 417 [45753:46169](-) rps14 303 [23805:24107](-) rps14_copy2 120 [37704:37823](-) rps15 264 [78963:79226](+) rps15_copy2 264 [82982:83245](-) rps16 234 [6001:6040](-) [4930:5123](-) rps18 306 [40870:41175](+) rps19 279 [50566:50844](-) rps19_copy2 279 [111364:111642](+) rps2 738 [14598:15335](-) rps3 651 [49358:50008](-) rps4 606 [27863:28468](-) rps7 468 [61518:61985](-) rps7_copy2 468 [100223:100690](+) rps8 393 [46836:47228](-) ycf1 5295 [73294:78588](+) ycf15 198 [59920:60117](+) ycf15_copy2 198 [102091:102288](-) ycf1_copy2 5295 [83620:88914](-) ycf2 6753 [53030:59782](+) ycf2_copy2 6753 [102426:109178](-) 35 tRNA genes (25 are unique) Genes Location trnH-GUG [1:74](-) trnK-UUU [3985:4021](-) [1484:1519](-) trnQ-UUG [7195:7266](-) trnS-GCU [8056:8143](-) trnS-CGA [8414:8445](+) [9139:9199](+) trnR-UCU [9350:9421](+) trnC-GCA [18147:18217](+) trnD-GUC [19350:19423](-) trnY-GUA [19551:19634](-) trnE-UUC [19698:19770](-) trnT-GGU [20395:20466](+) trnS-UGA [22994:23086](-) trnG-GCC [23460:23530](+) trnM-CAU [23596:23669](-) trnS-GGA [27504:27590](+) trnT-UGU [28701:28773](-) trnL-UAA [28829:28863](+) [29345:29394](+) trnF-GAA [29743:29815](+) trnM-CAU_copy2 [30703:30775](+) trnW-CCA [39336:39409](-) trnP-UGG [39579:39652](-) trnM-CAU_copy3 [52864:52937](-) trnL-CAA [60460:60540](-) trnV-GAC [64430:64501](+) trnE-UUC_copy2 [66526:66557](+) [67499:67538](+) trnR-ACG [72269:72342](+) trnN-GUU [72888:72959](-) trnL-UAG [80536:80615](-) trnL-UAG_copy2 [81593:81672](+) trnN-GUU_copy2 [89249:89320](+) trnR-ACG_copy2 [89866:89939](-) trnE-UUC_copy3 [95651:95682](-) [94670:94709](-) trnV-GAC_copy2 [97707:97778](-) trnL-CAA_copy2 [101668:101748](+) trnM-CAU_copy4 [109271:109344](+) 6 rRNA genes (3 are unique) Genes Location rrn16S [64729:66218](+) rrn23S [68670:71476](+) rrn5S [71915:72045](+) rrn5S_copy2 [90163:90293](-) rrn23S_copy2 [90732:93538](-) rrn16S_copy2 [95990:97479](-) This genome has 10 genes with intron(s). Details are as follows: 9 gene(s) have 1 intron(s) Genes Exons rps16 [6001:6040](-) [4930:5123](-) rpl16 [49176:49184](-) [47883:48284](-) rpl2 [52012:52402](-) [50911:51341](-) rpl2_copy2 [109806:110196](+) [110867:111297](+) trnK-UUU [3985:4021](-) [1484:1519](-) trnS-CGA [8414:8445](+) [9139:9199](+) trnL-UAA [28829:28863](+) [29345:29394](+) trnE-UUC_copy2 [66526:66557](+) [67499:67538](+) trnE-UUC_copy3 [95651:95682](-) [94670:94709](-) 1 gene(s) have 2 intron(s) Genes Exons clpP [44704:44774](-) [43671:43964](-) [42804:43029](-)