1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhB, ndhC, ndhJ, ndhK Subunits of cytochrome b/f complex petB, petD, petG, petL Subunits of photosystem I psaA, psaB, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbJ, psbL, psbM, psbN, psbT, psbZ Large subunit of ribosome rpl14, rpl16, rpl20, rpl23, rpl33, rpl36 Small subunit of ribosome rps12, rps14, rps18, rps19, rps4, rps8 DNA dependent RNA polymerase rpoA Subunit of rubisco rbcL c-type cytochrom synthesis gene None Envelop membrane protein cemA Maturase None Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA Subunits of NADH-dehydrogenase ndhA, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI Subunits of cytochrome b/f complex petA, petN Subunits of photosystem I psaC Subunits of photosystem II psbI, psbK, ycf3 Large subunit of ribosome rpl2, rpl22, rpl32 Small subunit of ribosome rps11, rps15, rps16, rps2, rps3, rps7 DNA dependent RNA polymerase rpoB, rpoC1, rpoC2 Subunit of rubisco None c-type cytochrom synthesis gene ccsA Envelop membrane protein None Maturase matK Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf1, ycf15, ycf2 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [2742:3017](-) [1617:2069](-) clpP (3/3) [57782:57852](-) [56786:57076](-) [55990:56230](-) ndhB (1/2) [72650:73453](+) petB (1/2) [61617:62336](+) petD (1/2) [63113:63643](+) rpl16 (1/2) [67376:67807](-) rps12 (3/3) [55610:55723](-) [83454:83685](+) [84263:84288](+) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon atpF ATA atpH TTG atpI ATA psbM ATT psbC GTG psaB TTG rps4 TTG ndhK TTG accD ATT cemA GTG psbL GTG rps12 GTG psbB ATA psbT ATT psbH ATA rpl36 ATT rps8 ATA rpl14 ATA rpl16 ATA rps19 ATA rpl23 ATT 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 73 genes (72 unique genes). Including: 48 protein-coding genes (48 are unique) 21 tRNA genes (20 are unique) 4 rRNA genes (4 are unique) Details are as follows: 48 protein-coding genes (48 are unique) Genes Length Location accD 939 [44378:45316](+) atpB 1482 [40306:41787](-) atpE 399 [39902:40300](-) atpF 729 [2742:3017](-) [1617:2069](-) atpH 237 [3254:3490](-) atpI 660 [4275:4934](-) cemA 846 [47566:48411](+) clpP 603 [57782:57852](-) [56786:57076](-) [55990:56230](-) ndhB 804 [72650:73453](+) ndhC 417 [37831:38247](-) ndhJ 513 [36568:37080](-) ndhK 930 [37157:38086](-) petB 720 [61617:62336](+) petD 531 [63113:63643](+) petG 117 [52077:52193](+) petL 96 [51832:51927](+) psaA 2253 [28869:31121](-) psaB 2217 [26639:28855](-) psaI 111 [45652:45762](+) psaJ 129 [53166:53294](+) psbA 1062 [85323:86384](+) psbB 1635 [58251:59885](+) psbC 1452 [21657:23108](-) psbD 1062 [23026:24087](-) psbE 252 [50839:51090](-) psbF 120 [50704:50823](-) psbH 180 [60590:60769](+) psbJ 126 [50323:50448](-) psbL 132 [50566:50697](-) psbM 123 [17898:18020](+) psbN 138 [60323:60460](-) psbT 120 [60071:60190](+) psbZ 189 [20690:20878](-) rbcL 1428 [42426:43853](+) rpl14 429 [66762:67190](-) rpl16 432 [67376:67807](-) rpl20 357 [54286:54642](-) rpl23 270 [71769:72038](-) rpl33 207 [53536:53742](+) rpl36 108 [65528:65635](-) rpoA 1002 [64022:65023](-) rps12 372 [55610:55723](-) [83454:83685](+) [84263:84288](+) rps14 303 [26103:26405](-) rps18 258 [53792:54049](+) rps19 234 [69828:70061](-) rps4 597 [34104:34700](-) rps8 363 [66152:66514](-) ycf4 555 [46177:46731](+) 21 tRNA genes (20 are unique) Genes Location trnS-ACU [8358:8394](+) [8691:8745](+) trnD-GUC [17183:17258](+) trnY-GUA [17309:17392](-) trnE-UUC [17407:17480](-) trnC-GCA [19783:19856](-) trnG-GCC [20364:20434](-) trnS-UGA [21258:21349](+) trnT-GGU [25345:25418](-) trnfM-CAU [25888:25961](-) trnS-GGA [33586:33674](+) trnL-CAA [35341:35375](+) [35955:36007](+) trnF-GAA [36278:36350](+) trnM-CAU [39405:39437](-) [38748:38787](-) trnM-CAU_copy2 [39683:39755](+) trnW-CCA [52443:52517](-) trnP-UGG [52701:52776](-) trnI-CAU [72200:72273](-) trnR-ACG [74946:75020](-) trnA-UGC [79714:79750](-) [78869:78903](-) trnH-GUG [86800:86874](+) trnN-GUU [94002:94075](-) 4 rRNA genes (4 are unique) Genes Location rrn5S [75263:75383](-) rrn4.5S [75650:75752](-) rrn23S [75877:78694](-) rrn16S [80077:81576](-) This genome has 7 genes with intron(s). Details are as follows: 5 gene(s) have 1 intron(s) Genes Exons atpF [2742:3017](-) [1617:2069](-) trnS-ACU [8358:8394](+) [8691:8745](+) trnL-CAA [35341:35375](+) [35955:36007](+) trnM-CAU [39405:39437](-) [38748:38787](-) trnA-UGC [79714:79750](-) [78869:78903](-) 2 gene(s) have 2 intron(s) Genes Exons rps12 [55610:55723](-) [83454:83685](+) [84263:84288](+) clpP [57782:57852](-) [56786:57076](-) [55990:56230](-)