1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl23, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf1, ycf2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome rpl22 Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [47695:47839](+) [48534:48946](+) clpP (1/3) [66406:66999](-) ndhA (2/2) [110615:111167](+) [112378:112916](+) ndhB (2/2) [99512:100234](+) [100913:101674](+) petB (2/2) [70157:70162](+) [70876:71517](+) petD (2/2) [71711:71718](+) [72431:72905](+) rpl16 (2/2) [78087:78095](-) [76627:77025](-) rpl2 (2/2) [80633:81020](-) [79502:79935](-) rpoC1 (2/2) [37107:37538](+) [38286:39908](+) rps12 (2/3) [60395:60508](-) [98476:98733](+) ycf3 (3/3) [16313:16436](+) [17158:17387](+) [18157:18309](+) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbL ACG rps8 GTG ndhD ACG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 109 genes (109 unique genes). Including: 75 protein-coding genes (75 are unique) 30 tRNA genes (30 are unique) 4 rRNA genes (4 are unique) Details are as follows: 75 protein-coding genes (75 are unique) Genes Length Location atpA 1536 [49025:50560](+) atpB 1467 [7416:8882](+) atpE 402 [8905:9306](+) atpF 558 [47695:47839](+) [48534:48946](+) atpH 246 [47052:47297](+) atpI 744 [45398:46141](+) ccsA 972 [117418:118389](-) cemA 690 [62237:62926](-) clpP 594 [66406:66999](-) matK 1512 [1920:3431](-) ndhA 1092 [110615:111167](+) [112378:112916](+) ndhB 1485 [99512:100234](+) [100913:101674](+) ndhC 363 [10647:11009](+) ndhD 1497 [115696:117192](+) ndhE 306 [114721:115026](+) ndhF 2235 [119795:122029](+) ndhG 531 [113980:114510](+) ndhH 1182 [109432:110613](+) ndhI 513 [112996:113508](+) ndhJ 477 [11857:12333](+) ndhK 675 [11052:11726](+) petA 963 [61076:62038](-) petB 648 [70157:70162](+) [70876:71517](+) petD 483 [71711:71718](+) [72431:72905](+) petG 114 [64585:64698](-) petL 96 [64860:64955](-) petN 90 [31688:31777](-) psaA 2274 [19189:21462](+) psaB 2205 [21488:23692](+) psaC 246 [115323:115568](+) psaI 105 [64124:64228](-) psaJ 135 [57491:57625](+) psbA 1062 [318:1379](-) psbB 1527 [67452:68978](+) psbC 1422 [26054:27475](-) psbD 1062 [27423:28484](-) psbE 252 [65468:65719](+) psbF 120 [65729:65848](+) psbH 222 [69802:70023](+) psbI 111 [52305:52415](-) psbJ 123 [66125:66247](+) psbK 186 [52727:52912](-) psbL 117 [65871:65987](+) psbM 105 [31109:31213](+) psbN 132 [69562:69693](-) psbT 108 [69111:69218](+) psbZ 189 [25245:25433](-) rbcL 1428 [5183:6610](-) rpl14 369 [76140:76508](-) rpl16 408 [78087:78095](-) [76627:77025](-) rpl2 822 [80633:81020](-) [79502:79935](-) rpl20 417 [59195:59611](-) rpl23 387 [81025:81411](-) rpl32 180 [119010:119189](-) rpl33 201 [58101:58301](+) rpl36 114 [74707:74820](-) rpoA 969 [73114:74082](-) rpoB 3201 [33880:37080](+) rpoC1 2055 [37107:37538](+) [38286:39908](+) rpoC2 4107 [40045:44151](+) rps11 414 [74169:74582](-) rps12 372 [60395:60508](-) [98476:98733](+) rps14 303 [23828:24130](+) rps15 273 [109062:109334](+) rps18 405 [58602:59006](+) rps19 279 [79086:79364](-) rps2 696 [44468:45163](+) rps3 648 [78256:78903](-) rps4 645 [15117:15761](+) rps7 468 [98786:99253](+) rps8 405 [75242:75646](-) ycf1 5676 [102897:108572](+) ycf2 5820 [82134:87953](+) ycf3 507 [16313:16436](+) [17158:17387](+) [18157:18309](+) ycf4 606 [63212:63817](-) 30 tRNA genes (30 are unique) Genes Location trnH-GUG [6:79](-) trnK-UUU [4157:4193](-) [1672:1706](-) trnM-CAU [9408:9480](-) trnV-UAC [9643:9681](+) [10263:10299](+) trnF-GAA [12939:13011](-) trnL-UAA [13715:13749](-) [13243:13292](-) trnT-UGU [14761:14833](+) trnS-GGA [15965:16052](-) trnfM-CAU [24294:24367](+) trnG-GCC [24501:24573](-) trnS-UGA [25783:25871](+) trnT-GGU [29475:29548](-) trnE-UUC [30028:30100](+) trnY-GUA [30175:30258](+) trnD-GUC [30657:30730](+) trnC-GCA [32600:32670](-) trnR-UCU [50675:50746](-) trnG-UCC [51594:51616](-) [50879:50926](-) trnS-GCU [52086:52172](+) trnQ-UUG [53264:53335](+) trnW-CCA [56700:56773](-) trnP-UGG [56949:57022](-) trnI-CAU [81745:81818](-) trnL-CAA [88503:88583](-) trnR-ACG [88972:89045](-) trnA-UGC [93619:93656](-) [92784:92818](-) trnI-GAU [94711:94747](-) [93721:93755](-) trnV-GAC [96690:96761](-) trnN-GUU [102361:102432](-) trnL-UAG [118509:118588](-) 4 rRNA genes (4 are unique) Genes Location rrn5 [89271:89391](-) rrn4.5 [89615:89717](-) rrn23 [89815:92612](-) rrn16 [94970:96461](-) This genome has 16 genes with intron(s). Details are as follows: 15 gene(s) have 1 intron(s) Genes Exons rpoC1 [37107:37538](+) [38286:39908](+) atpF [47695:47839](+) [48534:48946](+) rps12 [60395:60508](-) [98476:98733](+) petB [70157:70162](+) [70876:71517](+) petD [71711:71718](+) [72431:72905](+) rpl16 [78087:78095](-) [76627:77025](-) rpl2 [80633:81020](-) [79502:79935](-) ndhB [99512:100234](+) [100913:101674](+) ndhA [110615:111167](+) [112378:112916](+) trnK-UUU [4157:4193](-) [1672:1706](-) trnV-UAC [9643:9681](+) [10263:10299](+) trnL-UAA [13715:13749](-) [13243:13292](-) trnG-UCC [51594:51616](-) [50879:50926](-) trnA-UGC [93619:93656](-) [92784:92818](-) trnI-GAU [94711:94747](-) [93721:93755](-) 1 gene(s) have 2 intron(s) Genes Exons ycf3 [16313:16436](+) [17158:17387](+) [18157:18309](+)