1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps15, rps15_copy2, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf1, ycf2, ycf2_copy2, ycf4 Other genes chlB, chlL, chlL_copy2, chlN, chlN_copy2 Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbI Large subunit of ribosome rpl23, rpl32 Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location rps12 375 join{[46818:46932](-), [100573:100801](+), [101277:101310](+)} rps12_copy2 378 join{[46818:46932](+), [69355:69587](-), [68850:68882](-)} 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [29911:30055](+) [30639:31048](+) clpP (1/3) [47066:47731](-) petB (2/2) [51205:51210](+) [51727:52368](+) petD (2/2) [52506:52513](+) [53037:53514](+) rpl16 (2/2) [57360:57368](-) [56362:56757](-) rpl2 (2/2) [59849:60209](-) [58911:59350](-) rpoC1 (2/2) [21195:21652](+) [22233:23862](+) rps12 (3/3) [46819:46932](-) [100574:100801](+) [101278:101310](+) rps12_copy2 (3/3) [46819:46932](+) [69356:69587](-) [68851:68882](-) ycf3 (3/3) [15845:15970](-) [15003:15230](-) [14192:14344](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon rps12_copy2 ATA rpl22 GTG chlL GTG chlL_copy2 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 117 genes (90 unique genes). Including: 72 protein-coding genes (66 are unique) 37 tRNA genes (20 are unique) 8 rRNA genes (4 are unique) Details are as follows: 72 protein-coding genes (66 are unique) Genes Length Location atpA 1524 [31092:32615](+) atpB 1470 [36758:38227](-) atpE 408 [36297:36704](-) atpF 555 [29911:30055](+) [30639:31048](+) atpH 246 [29445:29690](+) atpI 756 [28566:29321](+) ccsA 924 [81610:82533](-) cemA 756 [41168:41923](+) chlB 1545 [3671:5215](-) chlL 870 [78355:79224](+) chlL_copy2 870 [90937:91806](-) chlN 1407 [79243:80649](+) chlN_copy2 1407 [89512:90918](-) clpP 666 [47066:47731](-) infA 234 [55127:55360](-) matK 1662 [1399:3060](-) petA 966 [42064:43029](+) petB 648 [51205:51210](+) [51727:52368](+) petD 486 [52506:52513](+) [53037:53514](+) petG 114 [44449:44562](+) petL 96 [44241:44336](+) petN 90 [17408:17497](-) psaA 2253 [11575:13827](-) psaB 2205 [9353:11557](-) psaC 246 [81259:81504](+) psaI 111 [40310:40420](+) psaJ 132 [45002:45133](+) psbA 1062 [1:1062](-) psbB 1527 [48900:50426](+) psbC 1422 [6653:8074](+) psbD 1062 [5644:6705](+) psbE 252 [43649:43900](-) psbF 120 [43520:43639](-) psbH 228 [50887:51114](+) psbJ 123 [43149:43271](-) psbK 165 [33741:33905](-) psbL 117 [43392:43508](-) psbM 105 [17206:17310](+) psbN 132 [50658:50789](-) psbT 114 [50492:50605](+) psbZ 186 [8382:8567](+) rbcL 1428 [38582:40009](+) rpl14 369 [55918:56286](-) rpl16 405 [57360:57368](-) [56362:56757](-) rpl2 801 [59849:60209](-) [58911:59350](-) rpl20 348 [45869:46216](-) rpl22 429 [58111:58539](-) rpl33 171 [45251:45421](+) rpl36 114 [54897:55010](-) rpoA 777 [53625:54401](-) rpoB 3303 [17900:21202](+) rpoC1 2088 [21195:21652](+) [22233:23862](+) rpoC2 3696 [23960:27655](+) rps11 399 [54413:54811](-) rps12 375 [46819:46932](-) [100574:100801](+) [101278:101310](+) rps12_copy2 378 [46819:46932](+) [69356:69587](-) [68851:68882](-) rps14 294 [8952:9245](-) rps15 231 [80724:80954](+) rps15_copy2 231 [89207:89437](-) rps18 294 [45492:45785](+) rps19 345 [58551:58895](-) rps2 720 [27728:28447](+) rps3 648 [57474:58121](-) rps4 630 [16244:16873](-) rps7 471 [68331:68801](-) rps7_copy2 471 [101360:101830](+) rps8 387 [55408:55794](-) ycf1 6057 [83080:89136](-) ycf2 6378 [61443:67820](+) ycf2_copy2 6378 [102341:108718](-) ycf3 507 [15845:15970](-) [15003:15230](-) [14192:14344](-) ycf4 558 [40544:41101](+) 37 tRNA genes (20 are unique) Genes Location trnK [3541:3578](-) [1209:1243](-) trnT [5371:5442](+) trnS [8159:8245](-) trnG [8708:8778](+) trnfM [8795:8868](-) trnS_copy2 [16107:16193](+) trnC [17629:17700](-) trnR [32616:32687](-) trnS_copy3 [33456:33544](+) trnQ [34120:34192](+) trnE [34302:34374](+) trnY [34413:34496](+) trnD [34603:34676](+) trnT_copy2 [34825:34897](-) trnL [35050:35084](+) [35375:35424](+) trnF [35551:35623](+) trnfM_copy2 [36161:36233](+) trnR_copy2 [40115:40188](+) trnW [44586:44659](-) trnP [44732:44806](-) trnI [60792:60865](-) trnH [61119:61192](+) trnL_copy2 [68095:68174](-) trnV [70330:70401](+) trnI_copy2 [72283:72319](+) [73080:73114](+) trnA [73163:73202](+) [73961:73996](+) trnR_copy3 [77681:77754](+) trnN [77974:78047](+) trnL_copy3 [82595:82674](-) trnN_copy2 [92114:92187](-) trnR_copy4 [92407:92480](-) trnA_copy2 [96959:96998](-) [96165:96200](-) trnI_copy3 [97842:97878](-) [97047:97081](-) trnV_copy2 [99760:99831](-) trnL_copy4 [101987:102066](+) trnH_copy2 [108969:109042](-) trnI_copy4 [109296:109369](+) 8 rRNA genes (4 are unique) Genes Location rrn16 [70568:72049](+) rrn23 [74111:76952](+) rrn4.5 [77014:77116](+) rrn5 [77371:77514](+) rrn5_copy2 [92647:92790](-) rrn4.5_copy2 [93045:93147](-) rrn23_copy2 [93209:96050](-) rrn16_copy2 [98112:99593](-) This genome has 15 genes with intron(s). Details are as follows: 12 gene(s) have 1 intron(s) Genes Exons rpoC1 [21195:21652](+) [22233:23862](+) atpF [29911:30055](+) [30639:31048](+) petB [51205:51210](+) [51727:52368](+) petD [52506:52513](+) [53037:53514](+) rpl16 [57360:57368](-) [56362:56757](-) rpl2 [59849:60209](-) [58911:59350](-) trnK [3541:3578](-) [1209:1243](-) trnL [35050:35084](+) [35375:35424](+) trnI_copy2 [72283:72319](+) [73080:73114](+) trnA [73163:73202](+) [73961:73996](+) trnA_copy2 [96959:96998](-) [96165:96200](-) trnI_copy3 [97842:97878](-) [97047:97081](-) 3 gene(s) have 2 intron(s) Genes Exons ycf3 [15845:15970](-) [15003:15230](-) [14192:14344](-) rps12 [46819:46932](-) [100574:100801](+) [101278:101310](+) rps12_copy2 [46819:46932](+) [69356:69587](-) [68851:68882](-)