1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbI_copy2, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf12, ycf12_copy2, ycf2, ycf4 Other genes chlB, chlL, chlN, psaM, psaM_copy2 Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome None Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [82710:82854](-) [81526:81935](-) clpP (1/3) [118622:119212](-) petB (2/2) [4153:4158](+) [4947:5594](+) petD (2/2) [5808:5816](+) [6513:7052](+) rpl16 (2/2) [11680:11688](-) [10399:10797](-) rpl2 (2/2) [14325:14724](-) [13215:13645](-) rpoC1 (2/2) [91973:92404](-) [89647:91305](-) rps12 (3/3) [118341:118454](-) [58673:58904](+) [59448:59473](+) ycf3 (3/3) [20382:20510](+) [21243:21470](+) [22184:22339](+) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon rpl22 GTG chlL GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 114 genes (97 unique genes). Including: 75 protein-coding genes (72 are unique) 35 tRNA genes (21 are unique) 4 rRNA genes (4 are unique) Details are as follows: 75 protein-coding genes (72 are unique) Genes Length Location accD 969 [107066:108034](+) atpA 1524 [79959:81482](-) atpB 1479 [102834:104312](-) atpE 411 [102415:102825](-) atpF 555 [82710:82854](-) [81526:81935](-) atpH 246 [83188:83433](-) atpI 747 [83935:84681](-) ccsA 960 [53660:54619](-) cemA 786 [110354:111139](+) chlB 1545 [74695:76239](-) chlL 876 [42305:43180](+) chlN 1413 [43297:44709](+) clpP 591 [118622:119212](-) infA 237 [8995:9231](-) matK 1548 [71525:73072](-) petA 960 [111364:112323](+) petB 654 [4153:4158](+) [4947:5594](+) petD 549 [5808:5816](+) [6513:7052](+) petG 114 [115127:115240](+) petL 141 [114834:114974](+) petN 90 [96447:96536](+) psaA 2253 [22945:25197](+) psaB 2205 [25223:27427](+) psaC 246 [52738:52983](+) psaI 111 [108517:108627](+) psaJ 132 [115999:116130](+) psaM 93 [732:824](+) psaM_copy2 93 [78029:78121](-) psbA 1062 [69703:70764](-) psbB 1527 [1775:3301](+) psbC 1422 [29310:30731](-) psbD 1062 [30679:31740](-) psbE 252 [113367:113618](-) psbF 120 [113238:113357](-) psbH 228 [3776:4003](+) psbI 111 [1199:1309](-) psbI_copy2 111 [77544:77654](+) psbJ 123 [112854:112976](-) psbK 180 [76879:77058](+) psbL 117 [113095:113211](-) psbM 114 [97400:97513](-) psbN 132 [3565:3696](-) psbT 108 [3376:3483](+) psbZ 189 [28561:28749](-) rbcL 1428 [105017:106444](+) rpl14 369 [9904:10272](-) rpl16 408 [11680:11688](-) [10399:10797](-) rpl2 831 [14325:14724](-) [13215:13645](-) rpl20 360 [117272:117631](-) rpl22 411 [12438:12848](-) rpl23 276 [14745:15020](-) rpl32 180 [56233:56412](-) rpl33 207 [116455:116661](+) rpl36 114 [8778:8891](-) rpoA 1008 [7236:8243](-) rpoB 3231 [92427:95657](-) rpoC1 2091 [91973:92404](-) [89647:91305](-) rpoC2 3651 [85903:89553](-) rps11 393 [8303:8695](-) rps12 372 [118341:118454](-) [58673:58904](+) [59448:59473](+) rps14 300 [27580:27879](+) rps15 267 [50797:51063](+) rps18 261 [116780:117040](+) rps19 279 [12879:13157](-) rps2 705 [84973:85677](-) rps3 654 [11782:12435](-) rps4 606 [18616:19221](+) rps7 468 [59526:59993](+) rps8 399 [9365:9763](-) ycf1 5526 [44947:50472](+) ycf12 102 [332:433](-) ycf12_copy2 102 [78420:78521](+) ycf2 6342 [62608:68949](-) ycf3 513 [20382:20510](+) [21243:21470](+) [22184:22339](+) ycf4 555 [109173:109727](+) 35 tRNA genes (21 are unique) Genes Location trnS [1022:1109](+) trnI [15398:15471](-) trnF [16836:16908](-) trnL [17818:17852](-) [17280:17329](-) trnT [18275:18347](+) trnS_copy2 [19535:19621](-) trnfM [28008:28081](+) trnG [28238:28308](-) trnS_copy3 [29036:29122](+) trnT_copy2 [33036:33107](-) trnI_copy2 [35406:35441](+) [36422:36456](+) trnA [36537:36573](+) [37349:37383](+) trnR [41120:41193](+) trnN [41932:42003](-) trnL_copy2 [54755:54834](-) trnP [54990:55063](+) trnV [57402:57473](-) trnL_copy3 [61987:62067](+) trnH [69152:69225](-) trnI_copy3 [69483:69556](+) trnK [73855:73891](-) [71311:71342](-) trnQ [76449:76520](-) trnS_copy4 [77744:77831](-) trnG_copy2 [78706:78728](+) [79519:79566](+) trnR_copy2 [79790:79861](+) trnC [96061:96131](+) trnD [98432:98505](-) trnY [98778:98861](-) trnE [98929:99001](-) trnT_copy3 [100375:100446](+) trnV_copy2 [101998:102036](-) [101419:101453](-) trnzM [102230:102302](+) trnR_copy3 [106734:106807](+) trnW [115364:115437](-) trnP_copy2 [115613:115686](-) 4 rRNA genes (4 are unique) Genes Location rrn16 [33549:35039](+) rrn23 [37532:40333](+) rrn4.5 [40442:40544](+) rrn5 [40788:40907](+) This genome has 14 genes with intron(s). Details are as follows: 12 gene(s) have 1 intron(s) Genes Exons petB [4153:4158](+) [4947:5594](+) petD [5808:5816](+) [6513:7052](+) rpl16 [11680:11688](-) [10399:10797](-) rpl2 [14325:14724](-) [13215:13645](-) atpF [82710:82854](-) [81526:81935](-) rpoC1 [91973:92404](-) [89647:91305](-) trnL [17818:17852](-) [17280:17329](-) trnI_copy2 [35406:35441](+) [36422:36456](+) trnA [36537:36573](+) [37349:37383](+) trnK [73855:73891](-) [71311:71342](-) trnG_copy2 [78706:78728](+) [79519:79566](+) trnV_copy2 [101998:102036](-) [101419:101453](-) 2 gene(s) have 2 intron(s) Genes Exons ycf3 [20382:20510](+) [21243:21470](+) [22184:22339](+) rps12 [118341:118454](-) [58673:58904](+) [59448:59473](+)