1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhH_copy2, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps12_copy3, rps14, rps15, rps15_copy2, rps16, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf15, ycf15_copy2, ycf2, ycf2_copy2, ycf4, ycf68, ycf68_copy2 Other genes ihbA, orf188, orf42, orf42_copy2, orf56, orf56_copy2 Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbZ Large subunit of ribosome None Small subunit of ribosome None DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf1 Other genes None 2. Multicopy genes with different lengths: Genes Length Location rps12 129 [71884:72013](-) rps12_copy2 342 [99359:99701](-) rps12_copy3 342 [149418:149760](+) 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [13076:13234](-) [11975:12412](-) clpP (2/3) [74125:74193](-) [73028:73330](-) ndhA (2/2) [130517:131074](-) [128536:129075](-) ndhB (2/2) [97233:98009](-) [95812:96561](-) ndhB_copy2 (2/2) [151111:151887](+) [152559:153308](+) petB (2/2) [77221:77229](+) [77970:78623](+) petD (1/2) [79488:80012](+) rpl16 (2/2) [84933:84941](-) [83480:83917](-) rpl2 (1/2) [86622:87437](-) rpoC1 (2/2) [23662:24117](-) [21224:22843](-) rps12 (1/3) [71885:72013](-) rps12_copy2 (1/3) [99360:99701](-) rps12_copy3 (1/3) [149419:149760](+) rps16 (2/2) [5945:5984](-) [4906:5126](-) ycf3 (3/3) [45908:46033](-) [44965:45192](-) [44044:44196](-) ### The exon column shows the number of (actual exons/expected exons). ### Uncommon intron-contained genes: ycf68 2 [102360:102368](+) [103475:103816](+)] orf56 2 [105762:105788](-) [104857:104976](-)] orf56_copy2 2 [143332:143358](+) [144144:144263](+)] ycf68_copy2 2 [146752:146760](-) [145304:145645](-)] 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon petN ATT psbT ATC rpl22 GTG rps19 GTG ycf15 ATT ycf15_copy2 ATT 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 133 genes (113 unique genes). Including: 93 protein-coding genes (82 are unique) 40 tRNA genes (31 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 16S ribosomal RNA [146122:147611](-) 23S ribosomal RNA [140914:143718](-) 4.5S ribosomal RNA [140710:140813](-) 5S ribosomal RNA [140355:140474](-) 93 protein-coding genes (82 are unique) Genes Length Location accD 1548 [59128:60675](+) atpA 1524 [10396:11919](-) atpB 1473 [54926:56398](-) atpE 402 [54502:54903](-) atpF 597 [13076:13234](-) [11975:12412](-) atpH 246 [13589:13834](-) atpI 744 [14950:15693](-) ccsA 954 [123182:124135](+) cemA 690 [63117:63806](+) clpP 372 [74125:74193](-) [73028:73330](-) ihbA 189 [37307:37495](+) matK 1509 [1977:3485](-) ndhA 1098 [130517:131074](-) [128536:129075](-) ndhB 1527 [97233:98009](-) [95812:96561](-) ndhB_copy2 1527 [151111:151887](+) [152559:153308](+) ndhC 363 [52111:52473](-) ndhD 1512 [124422:125933](-) ndhE 306 [126560:126865](-) ndhF 2133 [119098:121230](-) ndhG 531 [127099:127629](-) ndhH 1182 [116847:118028](+) ndhH_copy2 1182 [131076:132257](-) ndhI 504 [127951:128454](-) ndhJ 498 [50759:51256](-) ndhK 894 [51338:52231](-) orf188 546 [118030:118575](+) orf42 129 [104528:104656](-) orf42_copy2 129 [144464:144592](+) orf56 147 [105762:105788](-) [104857:104976](-) orf56_copy2 147 [143332:143358](+) [144144:144263](+) petA 963 [64034:64996](+) petB 663 [77221:77229](+) [77970:78623](+) petD 525 [79488:80012](+) petG 114 [68095:68208](+) petL 96 [67772:67867](+) petN 96 [29606:29701](+) psaA 2253 [40992:43244](-) psaB 2205 [38761:40965](-) psaC 246 [126049:126294](-) psaI 102 [61398:61499](+) psaJ 135 [69151:69285](+) psbA 1062 [375:1436](-) psbB 1527 [74711:76237](+) psbC 1422 [35197:36618](+) psbD 1062 [34188:35249](+) psbE 252 [66393:66644](-) psbF 120 [66261:66380](-) psbH 222 [76871:77092](+) psbI 111 [7872:7982](+) psbJ 123 [65865:65987](-) psbK 180 [7302:7481](+) psbL 117 [66122:66238](-) psbM 105 [30902:31006](-) psbN 132 [76623:76754](-) psbT 111 [76447:76557](+) rbcL 1464 [56801:58264](+) rpl14 369 [83008:83376](-) rpl16 447 [84933:84941](-) [83480:83917](-) rpl2 816 [86622:87437](-) rpl20 351 [70736:71086](-) rpl22 471 [85746:86216](-) rpl23 279 [87442:87720](-) rpl32 174 [121896:122069](+) rpl33 201 [69743:69943](+) rpl36 114 [81783:81896](-) rpoA 1014 [80185:81198](-) rpoB 3189 [24123:27311](-) rpoC1 2076 [23662:24117](-) [21224:22843](-) rpoC2 4206 [16856:21061](-) rps11 417 [81275:81691](-) rps12 129 [71885:72013](-) rps12_copy2 342 [99360:99701](-) rps12_copy3 342 [149419:149760](+) rps14 303 [38333:38635](-) rps15 273 [116452:116724](+) rps15_copy2 273 [132380:132652](-) rps16 261 [5945:5984](-) [4906:5126](-) rps18 348 [70134:70481](+) rps19 282 [86291:86572](-) rps2 711 [15931:16641](-) rps3 657 [85105:85761](-) rps4 612 [47255:47866](-) rps7 468 [98288:98755](-) rps7_copy2 468 [150365:150832](+) rps8 405 [82381:82785](-) ycf15 171 [100549:100719](-) ycf15_copy2 171 [148401:148571](+) ycf2 6627 [88233:94859](+) ycf2_copy2 6627 [154261:160887](-) ycf3 507 [45908:46033](-) [44965:45192](-) [44044:44196](-) ycf4 555 [61937:62491](+) ycf68 351 [102360:102368](+) [103475:103816](+) ycf68_copy2 351 [146752:146760](-) [145304:145645](-) 40 tRNA genes (31 are unique) Genes Location trnH-GUG [62:136](-) trnK-UUU [4240:4276](-) [1663:1697](-) trnQ-UUG [6860:6931](-) trnS-GCU [8121:8207](-) trnG-UCC [8733:8755](+) [9635:9682](+) trnR-UCU [10218:10289](+) trnC-GCA [28612:28683](+) trnD-GUC [31665:31738](-) trnY-GUA [31845:31928](-) trnE-UUC [31984:32056](-) trnT-GGU [32806:32877](+) trnM-CAU [32812:32870](+) trnS-UGA [36865:36953](-) trnG-GCC [37771:37840](+) trnfM-CAU [38118:38191](-) trnS-GGA [46859:46945](+) trnT-UGU [48305:48377](-) trnL-UAA [49260:49296](+) [49638:49687](+) trnF-GAA [50059:50131](+) trnV-UAC [54010:54047](-) [53389:53425](-) trnM-CAU_copy2 [54222:54294](+) trnT-GGU_copy2 [54228:54286](+) trnW-CCA [68342:68414](-) trnP-UGG [68580:68653](-) trnP-GGG [68582:68652](-) trnI-CAU [87915:87988](-) trnL-CAA [95205:95285](-) trnV-GAC [101215:101286](+) trnI-GAU [103296:103337](+) [104263:104297](+) trnA-UGC [104361:104398](+) [105210:105244](+) trnR-ACG [108988:109060](+) trnN-GUU [109621:109693](-) trnL-UAG [122977:123056](+) trnN-GUU_copy2 [139411:139483](+) trnR-ACG_copy2 [140044:140116](-) trnA-UGC_copy2 [144722:144759](-) [143876:143910](-) trnI-GAU_copy2 [145783:145824](-) [144823:144857](-) trnV-GAC_copy2 [147834:147905](-) trnL-CAA_copy2 [153835:153915](+) trnI-CAU_copy2 [161132:161205](+) 0 rRNA genes (0 are unique) Genes Location This genome has 22 genes with intron(s). Details are as follows: 21 gene(s) have 1 intron(s) Genes Exons rps16 [5945:5984](-) [4906:5126](-) atpF [13076:13234](-) [11975:12412](-) rpoC1 [23662:24117](-) [21224:22843](-) clpP [74125:74193](-) [73028:73330](-) petB [77221:77229](+) [77970:78623](+) rpl16 [84933:84941](-) [83480:83917](-) ndhB [97233:98009](-) [95812:96561](-) ycf68 [102360:102368](+) [103475:103816](+) orf56 [105762:105788](-) [104857:104976](-) ndhA [130517:131074](-) [128536:129075](-) orf56_copy2 [143332:143358](+) [144144:144263](+) ycf68_copy2 [146752:146760](-) [145304:145645](-) ndhB_copy2 [151111:151887](+) [152559:153308](+) trnK-UUU [4240:4276](-) [1663:1697](-) trnG-UCC [8733:8755](+) [9635:9682](+) trnL-UAA [49260:49296](+) [49638:49687](+) trnV-UAC [54010:54047](-) [53389:53425](-) trnI-GAU [103296:103337](+) [104263:104297](+) trnA-UGC [104361:104398](+) [105210:105244](+) trnA-UGC_copy2 [144722:144759](-) [143876:143910](-) trnI-GAU_copy2 [145783:145824](-) [144823:144857](-) 1 gene(s) have 2 intron(s) Genes Exons ycf3 [45908:46033](-) [44965:45192](-) [44044:44196](-)