1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf1, ycf1_copy2, ycf2, ycf2_copy2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbB, psbZ Large subunit of ribosome None Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [11545:11709](-) [10413:10823](-) clpP (3/3) [69068:69136](-) [67928:68236](-) [67106:67333](-) ndhA (2/2) [112164:112715](-) [110267:110806](-) ndhB (2/2) [87208:87929](-) [85774:86530](-) ndhB_copy2 (2/2) [129802:130524](+) [131202:131957](+) petB (2/2) [72065:72070](+) [72789:73436](+) petD (2/2) [73666:73673](+) [74400:74874](+) rpl16 (1/2) [78250:78660](-) rpl2 (2/2) [82679:83074](-) [81582:82016](-) rpl2_copy2 (2/2) [134657:135052](+) [135715:136149](+) rpoC1 (2/2) [21637:22065](-) [19265:20890](-) rps12 (3/3) [66856:66969](-) [89400:89631](-) [88837:88862](-) rps12_copy2 (3/3) [66856:66969](-) [128100:128331](+) [128869:128894](+) ycf3 (3/3) [43076:43201](-) [42107:42334](-) [41139:41294](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon rpl16 ATC rpl22 ATA rpl23 ATA ycf2 ATT ycf1 CTG ycf1_copy2 CTG ycf2_copy2 ATT rpl23_copy2 ATA 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 116 genes (102 unique genes). Including: 81 protein-coding genes (74 are unique) 35 tRNA genes (28 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 16S ribosomal RNA [124716:126207](-) 23S ribosomal RNA [119727:122532](-) 4.5S ribosomal RNA [119526:119628](-) 5S ribosomal RNA [119201:119322](-) 81 protein-coding genes (74 are unique) Genes Length Location atpA 1524 [8840:10363](-) atpB 1476 [51194:52669](-) atpE 402 [50769:51170](-) atpF 576 [11545:11709](-) [10413:10823](-) atpH 246 [12111:12356](-) atpI 744 [12865:13608](-) ccsA 957 [104856:105812](+) cemA 699 [58513:59211](+) clpP 606 [69068:69136](-) [67928:68236](-) [67106:67333](-) matK 1515 [2062:3576](-) ndhA 1092 [112164:112715](-) [110267:110806](-) ndhB 1479 [87208:87929](-) [85774:86530](-) ndhB_copy2 1479 [129802:130524](+) [131202:131957](+) ndhC 363 [48829:49191](-) ndhD 1521 [106112:107632](-) ndhE 306 [108311:108616](-) ndhF 2247 [100888:103134](-) ndhG 531 [108799:109329](-) ndhH 1182 [112717:113898](-) ndhI 495 [109648:110142](-) ndhJ 477 [47499:47975](-) ndhK 678 [48106:48783](-) petA 975 [59403:60377](+) petB 654 [72065:72070](+) [72789:73436](+) petD 483 [73666:73673](+) [74400:74874](+) petG 117 [63020:63136](+) petL 96 [62746:62841](+) petN 90 [26749:26838](+) psaA 2262 [37994:40255](-) psaB 2205 [35764:37968](-) psaC 246 [107778:108023](-) psaI 114 [57198:57311](+) psaJ 129 [64000:64128](+) psbA 1062 [339:1400](-) psbC 1422 [31584:33005](+) psbD 1062 [30575:31636](+) psbE 252 [61503:61754](-) psbF 120 [61374:61493](-) psbH 222 [71728:71949](+) psbI 111 [6334:6444](+) psbJ 123 [60943:61065](-) psbK 186 [5879:6064](+) psbL 117 [61230:61346](-) psbM 117 [27507:27623](-) psbN 132 [71480:71611](-) psbT 108 [71315:71422](+) rbcL 1428 [53414:54841](+) rpl14 369 [77779:78147](-) rpl16 411 [78250:78660](-) rpl2 831 [82679:83074](-) [81582:82016](-) rpl20 348 [65763:66110](-) rpl22 519 [80434:80952](-) rpl23 294 [83080:83373](-) rpl23_copy2 294 [134358:134651](+) rpl2_copy2 831 [134657:135052](+) [135715:136149](+) rpl32 168 [103836:104003](+) rpl33 201 [64723:64923](+) rpl36 114 [76767:76880](-) rpoA 1011 [75117:76127](-) rpoB 3213 [22092:25304](-) rpoC1 2055 [21637:22065](-) [19265:20890](-) rpoC2 4176 [14931:19106](-) rps11 417 [76205:76621](-) rps12 372 [66856:66969](-) [89400:89631](-) [88837:88862](-) rps12_copy2 372 [66856:66969](-) [128100:128331](+) [128869:128894](+) rps14 306 [35330:35635](-) rps15 273 [114043:114315](-) rps18 318 [65201:65518](+) rps19 279 [81036:81314](-) rps2 711 [13919:14629](-) rps3 648 [79780:80427](-) rps4 603 [43993:44595](-) rps7 468 [88316:88783](-) rps7_copy2 468 [128948:129415](+) rps8 405 [77205:77609](-) ycf1 552 [99634:100185](+) ycf1_copy2 552 [117546:118097](-) ycf2 693 [83994:84686](+) ycf2_copy2 693 [133045:133737](-) ycf3 510 [43076:43201](-) [42107:42334](-) [41139:41294](-) ycf4 555 [57678:58232](+) 35 tRNA genes (28 are unique) Genes Location trnH-GUG [109:183](-) trnK-UUU [4372:4408](-) [1762:1796](-) trnQ-UUG [5310:5381](-) trnS-GCU [6613:6700](-) trnR-UCU [8595:8666](+) trnC-GCA [26252:26323](+) trnD-GUC [28230:28303](-) trnY-GUA [28635:28718](-) trnE-UUC [28783:28855](-) trnT-GGU [29413:29484](+) trnS-UGA [33199:33286](-) trnG-UCC [34838:34908](+) trnfM-CAU [35102:35176](-) trnS-GGA [43579:43665](+) trnL-UAA [45763:45799](+) [46326:46375](+) trnF-GAA [46664:46736](+) trnV-UAC [50263:50300](-) [49641:49677](-) trnM-CAU [50502:50574](+) trnW-CCA [63258:63331](-) trnP-UGG [63464:63538](-) trnI-CAU [83580:83653](-) trnL-CAA [85168:85248](-) trnV-GAC [91209:91280](+) trnI-GAU [93313:93354](+) [94134:94168](+) trnA-UGC [94232:94269](+) [95002:95036](+) trnR-ACG [98568:98641](+) trnN-GUU [99206:99278](-) trnL-UAG [104667:104746](+) trnN-GUU_copy2 [118453:118525](+) trnR-ACG_copy2 [119090:119163](-) trnA-UGC_copy2 [123462:123499](-) [122695:122729](-) trnI-GAU_copy2 [124377:124418](-) [123563:123597](-) trnV-GAC_copy2 [126451:126522](-) trnL-CAA_copy2 [132483:132563](+) trnI-CAU_copy2 [134078:134151](+) 0 rRNA genes (0 are unique) Genes Location This genome has 20 genes with intron(s). Details are as follows: 16 gene(s) have 1 intron(s) Genes Exons atpF [11545:11709](-) [10413:10823](-) rpoC1 [21637:22065](-) [19265:20890](-) petB [72065:72070](+) [72789:73436](+) petD [73666:73673](+) [74400:74874](+) rpl2 [82679:83074](-) [81582:82016](-) ndhB [87208:87929](-) [85774:86530](-) ndhA [112164:112715](-) [110267:110806](-) ndhB_copy2 [129802:130524](+) [131202:131957](+) rpl2_copy2 [134657:135052](+) [135715:136149](+) trnK-UUU [4372:4408](-) [1762:1796](-) trnL-UAA [45763:45799](+) [46326:46375](+) trnV-UAC [50263:50300](-) [49641:49677](-) trnI-GAU [93313:93354](+) [94134:94168](+) trnA-UGC [94232:94269](+) [95002:95036](+) trnA-UGC_copy2 [123462:123499](-) [122695:122729](-) trnI-GAU_copy2 [124377:124418](-) [123563:123597](-) 4 gene(s) have 2 intron(s) Genes Exons rps12 [66856:66969](-) [89400:89631](-) [88837:88862](-) ycf3 [43076:43201](-) [42107:42334](-) [41139:41294](-) rps12_copy2 [66856:66969](-) [128100:128331](+) [128869:128894](+) clpP [69068:69136](-) [67928:68236](-) [67106:67333](-)