1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps15, rps16, rps18, rps19, rps19_copy2, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf2, ycf2_copy2, ycf4 Other genes lhbA Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbB, psbZ Large subunit of ribosome None Small subunit of ribosome None DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf1, ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location rps12 399 join{[70142:70256](-), [98390:98651](-), [97835:97859](-)} rps12_copy2 378 join{[70142:70256](-), [139230:139469](+), [140000:140025](+)} 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [13212:13372](-) [12078:12492](-) clpP (3/3) [72424:72492](-) [71268:71558](-) [70429:70656](-) ndhA (2/2) [120707:121258](-) [118961:119500](-) ndhB (2/2) [96211:96987](-) [94770:95525](-) ndhB_copy2 (2/2) [140874:141650](+) [142336:143091](+) petB (1/2) [76119:76769](+) petD (1/2) [77667:78191](+) rpl16 (1/2) [81380:81739](-) rpl2 (2/2) [85649:86041](-) [84528:84962](-) rpl2_copy2 (2/2) [151820:152212](+) [152899:153333](+) rpoC1 (2/2) [23237:23668](-) [20804:22423](-) rps12 (3/3) [70143:70256](-) [98391:98651](-) [97836:97859](-) rps12_copy2 (3/3) [70143:70256](-) [139231:139469](+) [140001:140025](+) rps16 (2/2) [6545:6588](-) [5445:5670](-) ycf3 (3/3) [44803:44928](-) [43822:44049](-) [42913:43068](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbL ACG petB TAT rps19 GTG rps19_copy2 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 119 genes (105 unique genes). Including: 83 protein-coding genes (76 are unique) 36 tRNA genes (29 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 16S ribosomal RNA [135927:137418](-) 23S ribosomal RNA [130702:133511](-) 4.5S ribosomal RNA [130500:130603](-) 5S ribosomal RNA [130115:130236](-) 83 protein-coding genes (76 are unique) Genes Length Location accD 1494 [57288:58781](+) atpA 1524 [10491:12014](-) atpB 1497 [52934:54430](-) atpE 402 [52536:52937](-) atpF 576 [13212:13372](-) [12078:12492](-) atpH 246 [13516:13761](-) atpI 744 [14513:15256](-) ccsA 963 [113767:114729](+) cemA 696 [61444:62139](+) clpP 588 [72424:72492](-) [71268:71558](-) [70429:70656](-) lhbA 189 [36314:36502](+) matK 1524 [2162:3685](-) ndhA 1092 [120707:121258](-) [118961:119500](-) ndhB 1533 [96211:96987](-) [94770:95525](-) ndhB_copy2 1533 [140874:141650](+) [142336:143091](+) ndhC 363 [50558:50920](-) ndhD 1503 [114985:116487](-) ndhE 306 [117165:117470](-) ndhF 2289 [110021:112309](-) ndhG 531 [117706:118236](-) ndhH 1182 [121260:122441](-) ndhI 516 [118361:118876](-) ndhJ 477 [49218:49694](-) ndhK 681 [49829:50509](-) petA 963 [62358:63320](+) petB 651 [76119:76769](+) petD 525 [77667:78191](+) petG 114 [66365:66478](+) petL 96 [66102:66197](+) petN 90 [29133:29222](+) psaA 2253 [39997:42249](-) psaB 2205 [37767:39971](-) psaC 246 [116635:116880](-) psaI 114 [59665:59778](+) psaJ 135 [67327:67461](+) psbA 1062 [509:1570](-) psbC 1422 [34281:35702](+) psbD 1062 [33272:34333](+) psbE 252 [64743:64994](-) psbF 120 [64614:64733](-) psbH 222 [75051:75272](+) psbI 111 [7984:8094](+) psbJ 123 [64217:64339](-) psbK 186 [7417:7602](+) psbL 117 [64475:64591](-) psbM 105 [30173:30277](-) psbN 132 [74815:74946](-) psbT 102 [74647:74748](+) rbcL 1428 [55214:56641](+) rpl14 369 [80871:81239](-) rpl16 360 [81380:81739](-) rpl2 828 [85649:86041](-) [84528:84962](-) rpl20 363 [68948:69310](-) rpl22 393 [83718:84110](-) rpl23 282 [86060:86341](-) rpl23_copy2 282 [151520:151801](+) rpl2_copy2 828 [151820:152212](+) [152899:153333](+) rpl32 159 [112621:112779](+) rpl33 201 [67927:68127](+) rpl36 114 [79977:80090](-) rpoA 1020 [78351:79370](-) rpoB 3213 [23695:26907](-) rpoC1 2052 [23237:23668](-) [20804:22423](-) rpoC2 4197 [16442:20638](-) rps11 417 [79436:79852](-) rps12 399 [70143:70256](-) [98391:98651](-) [97836:97859](-) rps12_copy2 378 [70143:70256](-) [139231:139469](+) [140001:140025](+) rps14 303 [37418:37720](-) rps15 273 [122545:122817](-) rps16 270 [6545:6588](-) [5445:5670](-) rps18 306 [68354:68659](+) rps19 279 [84188:84466](-) rps19_copy2 279 [153395:153673](+) rps2 711 [15474:16184](-) rps3 468 [82972:83439](-) rps4 609 [45916:46524](-) rps7 468 [97315:97782](-) rps7_copy2 468 [140079:140546](+) rps8 405 [80295:80699](-) ycf2 6864 [86671:93534](+) ycf2_copy2 6864 [144327:151190](-) ycf3 510 [44803:44928](-) [43822:44049](-) [42913:43068](-) ycf4 555 [60181:60735](+) 36 tRNA genes (29 are unique) Genes Location trnH-GUG [146:220](-) trnK-UUU [4441:4477](-) [1854:1882](-) trnQ-UUG [6989:7060](+) trnS-GCU [8250:8337](-) trnR-UCU [10279:10350](+) trnC-GCA [28124:28204](+) trnD-GUC [30796:30869](-) trnY-GUA [31199:31282](-) trnE-UUC [31341:31413](-) trnT-GGU [32037:32108](+) trnS-UGA [35936:36028](-) trnG-UCC [36949:37019](+) trnfM-CAU [37178:37251](-) trnS-GGA [45646:45732](+) trnT-UGU [46884:46956](-) trnL-UAA [47611:47647](+) [48154:48203](+) trnF-GAA [48475:48547](+) trnV-UAC [52009:52046](-) [51372:51408](-) trnM-CAU [52195:52266](+) trnW-CCA [66602:66675](-) trnP-GGG [66827:66900](-) trnI-CAU [86503:86576](-) trnL-CAA [94101:94181](-) trnV-GAC [100144:100215](+) trnI-GAU [102230:102271](+) [103212:103246](+) trnA-UGC [103311:103348](+) [104152:104186](+) trnR-ACG [108002:108075](+) trnN-GUU [108681:108753](-) trnL-UAG [113598:113677](+) trnN-GUU_copy2 [129108:129180](+) trnR-ACG_copy2 [129786:129859](-) trnA-UGC_copy2 [134513:134550](-) [133675:133709](-) trnI-GAU_copy2 [135590:135631](-) [134615:134649](-) trnV-GAC_copy2 [137646:137717](-) trnL-CAA_copy2 [143680:143760](+) trnI-CAU_copy2 [151285:151358](+) 0 rRNA genes (0 are unique) Genes Location This genome has 19 genes with intron(s). Details are as follows: 15 gene(s) have 1 intron(s) Genes Exons rps16 [6545:6588](-) [5445:5670](-) atpF [13212:13372](-) [12078:12492](-) rpoC1 [23237:23668](-) [20804:22423](-) rpl2 [85649:86041](-) [84528:84962](-) ndhB [96211:96987](-) [94770:95525](-) ndhA [120707:121258](-) [118961:119500](-) ndhB_copy2 [140874:141650](+) [142336:143091](+) rpl2_copy2 [151820:152212](+) [152899:153333](+) trnK-UUU [4441:4477](-) [1854:1882](-) trnL-UAA [47611:47647](+) [48154:48203](+) trnV-UAC [52009:52046](-) [51372:51408](-) trnI-GAU [102230:102271](+) [103212:103246](+) trnA-UGC [103311:103348](+) [104152:104186](+) trnA-UGC_copy2 [134513:134550](-) [133675:133709](-) trnI-GAU_copy2 [135590:135631](-) [134615:134649](-) 4 gene(s) have 2 intron(s) Genes Exons rps12 [70143:70256](-) [98391:98651](-) [97836:97859](-) ycf3 [44803:44928](-) [43822:44049](-) [42913:43068](-) rps12_copy2 [70143:70256](-) [139231:139469](+) [140001:140025](+) clpP [72424:72492](-) [71268:71558](-) [70429:70656](-)