1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl20, rpl22, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps16, rps18, rps2, rps3, rps4, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome rpl2, rpl23 Small subunit of ribosome rps19, rps7 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf15, ycf2 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [12529:12673](-) [11155:11564](-) clpP (3/3) [71947:72017](-) [70628:70919](-) [69723:69953](-) petB (2/2) [75628:75633](+) [76378:77019](+) petD (2/2) [77205:77212](+) [78079:78562](+) rpl16 (2/2) [83737:83745](-) [82126:82524](-) rpoC1 (2/2) [22479:22931](-) [20098:21705](-) rps12 (3/3) [69458:69568](-) [99766:99912](-) [99219:99266](-) rps16 (2/2) [4865:4904](-) [3726:3973](-) ycf3 (3/3) [44014:44137](-) [43064:43293](-) [42144:42296](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon matK ATT rpoC1 GTG rpl22 CTG ycf1 ATA 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 109 genes (102 unique genes). Including: 63 protein-coding genes (63 are unique) 38 tRNA genes (33 are unique) 8 rRNA genes (6 are unique) Details are as follows: 63 protein-coding genes (63 are unique) Genes Length Location accD 1449 [56514:57962](+) atpA 1524 [9559:11082](-) atpB 1503 [51592:53094](-) atpE 405 [51191:51595](-) atpF 555 [12529:12673](-) [11155:11564](-) atpH 246 [12804:13049](-) atpI 744 [13790:14533](-) ccsA 966 [114298:115263](+) cemA 690 [60603:61292](+) clpP 594 [71947:72017](-) [70628:70919](-) [69723:69953](-) infA 234 [80652:80885](-) matK 1536 [564:2099](-) petA 963 [61532:62494](+) petB 648 [75628:75633](+) [76378:77019](+) petD 492 [77205:77212](+) [78079:78562](+) petG 114 [65735:65848](+) petL 96 [65450:65545](+) petN 90 [28325:28414](+) psaA 2253 [39207:41459](-) psaB 2205 [36977:39181](-) psaC 246 [116776:117021](-) psaI 111 [58755:58865](+) psaJ 129 [66651:66779](+) psbA 1062 [152823:153884](-) psbB 1527 [72766:74292](+) psbC 1422 [33670:35091](+) psbD 1062 [32661:33722](+) psbE 252 [64027:64278](-) psbF 120 [63897:64016](-) psbH 222 [75230:75451](+) psbI 111 [7088:7198](+) psbJ 123 [63515:63637](-) psbK 186 [6430:6615](+) psbL 117 [63758:63874](-) psbM 105 [29220:29324](-) psbN 132 [74987:75118](-) psbT 102 [74815:74916](+) psbZ 189 [35550:35738](+) rbcL 1464 [54211:55674](+) rpl14 369 [81631:81999](-) rpl16 408 [83737:83745](-) [82126:82524](-) rpl20 384 [68323:68706](-) rpl22 312 [84666:84977](-) rpl32 174 [113144:113317](+) rpl33 201 [67369:67569](+) rpl36 114 [80424:80537](-) rpoA 1017 [78737:79753](-) rpoB 3213 [22937:26149](-) rpoC1 2061 [22479:22931](-) [20098:21705](-) rpoC2 4155 [15756:19910](-) rps11 417 [79837:80253](-) rps12 306 [69458:69568](-) [99766:99912](-) [99219:99266](-) rps14 303 [36530:36832](-) rps15 273 [120360:120632](-) rps16 288 [4865:4904](-) [3726:3973](-) rps18 306 [67760:68065](+) rps2 711 [14823:15533](-) rps3 657 [83931:84587](-) rps4 606 [45098:45703](-) rps8 396 [80999:81394](-) ycf1 5274 [120980:126253](-) ycf3 507 [44014:44137](-) [43064:43293](-) [42144:42296](-) ycf4 555 [59297:59851](+) 38 tRNA genes (33 are unique) Genes Location trnK(uuu) [3175:3211](-) [256:290](-) trnQ-UUG [5989:6060](-) trnS(gcu) [7346:7440](-) trnG(ucc) [8504:8534](+) [9214:9272](+) trnR(ucu) [9328:9400](+) trnC(gca) [27338:27408](+) trnD(guc) [30262:30335](-) trnY(gua) [30657:30740](-) trnE(uuc) [30800:30872](-) trnT(ggu) [31577:31648](+) trnS(uga) [35229:35321](-) trnG(gcc) [36044:36114](+) trnfM(cau) [36292:36365](-) trnS(gga) [44706:44792](+) trnT(ugu) [46039:46111](-) trnL(uaa) [46808:46842](+) [47631:47680](+) trnF(gaa) [48077:48149](+) trnV(uac) [50733:50771](-) [50123:50159](-) trnM(cau) [50947:51019](+) trnW(cca) [65967:66040](-) trnP(ugg) [66213:66286](-) trnH(gug) [85635:85708](+) trnl(cau) [87742:87815](-) trnL(caa) [95819:95899](-) trnV(gac) [102364:102435](+) trnl(gau) [104457:104493](+) [105444:105478](+) trnA(ugc) [105543:105580](+) [106382:106416](+) trnI(gau) [110137:110210](+) trnN(guu) [110767:110838](-) trnL(uag) [114078:114157](+) trnN(guu)_copy2 [126851:126922](+) trnR(acg) [127479:127552](-) trnA(ugc)_copy2 [132109:132146](-) [131273:131307](-) tenl(gau) [133196:133232](-) [132211:132245](-) trnV(gac)_copy2 [135254:135325](-) trnL(caa)_copy2 [141790:141870](+) trnI(cau) [149874:149947](+) trnH(gug)_copy2 [151981:152054](-) 8 rRNA genes (6 are unique) Genes Location rrn16 [102665:104154](+) rrn23 [106562:109367](+) rrn4.5 [109467:109569](+) rrn5 [109782:109902](+) rrn5rRNA [127787:127907](-) rnn4.5 [128120:128222](-) rrn23_copy2 [128322:131127](-) rrn16_copy2 [133535:135024](-) This genome has 17 genes with intron(s). Details are as follows: 14 gene(s) have 1 intron(s) Genes Exons rps16 [4865:4904](-) [3726:3973](-) atpF [12529:12673](-) [11155:11564](-) rpoC1 [22479:22931](-) [20098:21705](-) petB [75628:75633](+) [76378:77019](+) petD [77205:77212](+) [78079:78562](+) rpl16 [83737:83745](-) [82126:82524](-) trnK(uuu) [3175:3211](-) [256:290](-) trnG(ucc) [8504:8534](+) [9214:9272](+) trnL(uaa) [46808:46842](+) [47631:47680](+) trnV(uac) [50733:50771](-) [50123:50159](-) trnl(gau) [104457:104493](+) [105444:105478](+) trnA(ugc) [105543:105580](+) [106382:106416](+) trnA(ugc)_copy2 [132109:132146](-) [131273:131307](-) tenl(gau) [133196:133232](-) [132211:132245](-) 3 gene(s) have 2 intron(s) Genes Exons rps12 [69458:69568](-) [99766:99912](-) [99219:99266](-) ycf3 [44014:44137](-) [43064:43293](-) [42144:42296](-) clpP [71947:72017](-) [70628:70919](-) [69723:69953](-)