1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf2, ycf4 Other genes chlB, chlL, chlN, psaM Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome None Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [37588:37732](+) [38431:38840](+) ndhA (2/2) [114911:115459](-) [113600:114148](-) ndhB (2/2) [91300:92022](-) [89816:90571](-) petB (2/2) [61661:61666](-) [60237:60878](-) petD (2/2) [59996:60003](-) [58845:59355](-) rpl16 (2/2) [77022:77030](-) [75736:76155](-) rpl2 (2/2) [79750:80143](-) [78655:79085](-) rpoC1 (2/2) [28670:29110](+) [29801:31474](+) rps12 (3/3) [74293:74406](-) [93434:93665](-) [92832:92857](-) ycf3 (3/3) [8646:8771](+) [9455:9680](+) [10391:10548](+) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon rps12 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 110 genes (108 unique genes). Including: 81 protein-coding genes (81 are unique) 29 tRNA genes (27 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 16S ribosomal RNA [95187:96701](+) 23S ribosomal RNA [98941:101785](+) 4.5S ribosomal RNA [101786:101888](+) 5S ribosomal RNA [102103:102224](+) 81 protein-coding genes (81 are unique) Genes Length Location accD 2211 [52500:54710](+) atpA 1524 [38934:40457](+) atpB 1476 [48201:49676](+) atpE 408 [49686:50093](+) atpF 555 [37588:37732](+) [38431:38840](+) atpH 246 [37097:37342](+) atpI 747 [35735:36481](+) ccsA 957 [108127:109083](+) cemA 786 [66071:66856](+) chlB 1551 [5299:6849](-) chlL 873 [126979:127851](-) chlN 1374 [125584:126957](-) infA 243 [56591:56833](+) matK 1533 [1447:2979](-) ndhA 1098 [114911:115459](-) [113600:114148](-) ndhB 1479 [91300:92022](-) [89816:90571](-) ndhC 363 [45990:46352](-) ndhD 1506 [109328:110833](-) ndhE 303 [111610:111912](-) ndhF 2235 [103502:105736](-) ndhG 543 [112049:112591](-) ndhH 1182 [115461:116642](-) ndhI 492 [112951:113442](-) ndhJ 522 [44642:45163](-) ndhK 744 [45256:45999](-) petA 966 [67089:68054](+) petB 648 [61661:61666](-) [60237:60878](-) petD 519 [59996:60003](-) [58845:59355](-) petG 114 [70787:70900](+) petL 96 [70556:70651](+) petN 90 [23135:23224](+) psaA 2253 [11102:13354](+) psaB 2205 [13380:15584](+) psaC 246 [110977:111222](-) psaI 111 [64460:64570](+) psaJ 135 [71571:71705](+) psaM 90 [41200:41289](+) psbA 1062 [1:1062](-) psbB 1527 [62585:64111](-) psbC 1422 [17285:18706](-) psbD 1062 [18654:19715](-) psbE 258 [69023:69280](-) psbF 120 [68894:69013](-) psbH 228 [61814:62041](-) psbI 111 [41597:41707](-) psbJ 123 [68508:68630](-) psbK 186 [42352:42537](-) psbL 117 [68745:68861](-) psbM 105 [23974:24078](-) psbN 132 [62119:62250](+) psbT 108 [62331:62438](-) psbZ 189 [16672:16860](-) rbcL 1428 [50602:52029](+) rpl14 369 [75308:75676](-) rpl16 429 [77022:77030](-) [75736:76155](-) rpl2 825 [79750:80143](-) [78655:79085](-) rpl20 351 [73211:73561](-) rpl22 363 [77789:78151](-) rpl23 291 [80172:80462](-) rpl32 123 [106548:106670](+) rpl33 195 [72039:72233](+) rpl36 114 [56948:57061](+) rpoA 969 [57673:58641](+) rpoB 3279 [25367:28645](+) rpoC1 2115 [28670:29110](+) [29801:31474](+) rpoC2 3021 [31589:34609](+) rps11 447 [57168:57614](+) rps12 372 [74293:74406](-) [93434:93665](-) [92832:92857](-) rps14 303 [15733:16035](+) rps15 261 [116734:116994](-) rps18 390 [72629:73018](+) rps19 408 [78179:78586](-) rps2 702 [34805:35506](+) rps3 708 [77097:77804](-) rps4 624 [7696:8319](+) rps7 471 [92312:92782](-) rps8 348 [74779:75126](-) ycf1 6666 [117834:124499](-) ycf2 6810 [82204:89013](+) ycf3 510 [8646:8771](+) [9455:9680](+) [10391:10548](+) ycf4 555 [65137:65691](+) 29 tRNA genes (27 are unique) Genes Location trnK-UUU [3690:3726](-) [1229:1263](-) trnQ-UUG [7046:7117](-) trnT-UGU [7328:7400](+) trnfM-CAU [16178:16251](+) trnG-UCC [16367:16438](-) trnS-UGA [17018:17105](+) trnT-GGU [20854:20925](-) trnE-UUC [21802:21874](+) trnY-GUA [21943:22026](+) trnD-GUC [22222:22295](+) trnC-GCA [24583:24653](-) trnR-UCU [40589:40660](-) trnS-GCU [41461:41548](+) trnQ-UUG_copy2 [42880:42952](+) trnL-UAA [43212:43246](+) [43695:43744](+) trnF-GAA [44061:44133](+) trnV-UAC [47688:47725](-) [47161:47197](-) trnM-CAU [47856:47928](+) trnW-CCA [71017:71090](-) trnP-UGG [71211:71284](-) trnI-CAU [80792:80864](-) trnL-CAA [89227:89307](-) trnV-GAC [94619:94690](+) trnR-ACG [102342:102415](+) trnN-GUU [102884:102955](-) trnP-GGG [107643:107716](-) trnL-UAG [107875:107954](+) trnH-GUG [128059:128133](-) trnI-CAU_copy2 [128447:128519](+) 0 rRNA genes (0 are unique) Genes Location This genome has 13 genes with intron(s). Details are as follows: 11 gene(s) have 1 intron(s) Genes Exons rpoC1 [28670:29110](+) [29801:31474](+) atpF [37588:37732](+) [38431:38840](+) petD [59996:60003](-) [58845:59355](-) petB [61661:61666](-) [60237:60878](-) rpl16 [77022:77030](-) [75736:76155](-) rpl2 [79750:80143](-) [78655:79085](-) ndhB [91300:92022](-) [89816:90571](-) ndhA [114911:115459](-) [113600:114148](-) trnK-UUU [3690:3726](-) [1229:1263](-) trnL-UAA [43212:43246](+) [43695:43744](+) trnV-UAC [47688:47725](-) [47161:47197](-) 2 gene(s) have 2 intron(s) Genes Exons rps12 [74293:74406](-) [93434:93665](-) [92832:92857](-) ycf3 [8646:8771](+) [9455:9680](+) [10391:10548](+)