1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf2, ycf4 Other genes chlB, chlL, chlN Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome None Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [12970:13114](-) [11909:12318](-) ndhA (2/2) [49542:50099](-) [48243:48791](-) ndhB (2/2) [68019:68741](+) [69468:70223](+) petB (2/2) [101888:101893](+) [102642:103283](+) petD (2/2) [103444:103451](+) [104109:104598](+) rpl16 (2/2) [109485:109493](-) [108277:108687](-) rpl2 (2/2) [112288:112687](-) [111239:111669](-) rpoC1 (2/2) [22185:22626](-) [19767:21442](-) rps12 (3/3) [90457:90570](+) [66447:66678](+) [67218:67252](+) ycf3 (3/3) [40452:40580](-) [39513:39738](-) [38664:38821](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon rps12 GTG rps11 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 110 genes (109 unique genes). Including: 80 protein-coding genes (80 are unique) 30 tRNA genes (29 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 5S ribosomal RNA [56490:56611](-) 4.5S ribosomal RNA [56955:57051](-) 23S ribosomal RNA [57053:60029](-) 16S ribosomal RNA [63378:64890](-) 80 protein-coding genes (80 are unique) Genes Length Location accD 2424 [114118:116541](-) atpA 1524 [10185:11708](-) atpB 1476 [119563:121038](-) atpE 408 [119146:119553](-) atpF 555 [12970:13114](-) [11909:12318](-) atpH 246 [13412:13657](-) atpI 747 [14226:14972](-) ccsA 939 [86783:87721](-) cemA 786 [96792:97577](-) chlB 1542 [5198:6739](-) chlL 873 [126387:127259](-) chlN 1374 [124992:126365](-) infA 258 [106700:106957](-) matK 1518 [1771:3288](-) ndhA 1107 [49542:50099](-) [48243:48791](-) ndhB 1479 [68019:68741](+) [69468:70223](+) ndhC 363 [122850:123212](+) ndhD 1524 [44068:45591](-) ndhE 303 [46325:46627](-) ndhF 2229 [53120:55348](+) ndhG 546 [46750:47295](-) ndhH 1182 [50101:51282](-) ndhI 483 [47631:48113](-) ndhJ 522 [124039:124560](+) ndhK 801 [123182:123982](+) petA 966 [95680:96645](-) petB 648 [101888:101893](+) [102642:103283](+) petD 498 [103444:103451](+) [104109:104598](+) petG 114 [93603:93716](-) petL 96 [93826:93921](-) petN 90 [26848:26937](+) psaA 2250 [35878:38127](-) psaB 2205 [33652:35856](-) psaC 246 [45727:45972](-) psaI 111 [98959:99069](-) psaJ 129 [92851:92979](-) psbA 1062 [1:1062](-) psbB 1527 [99446:100972](+) psbC 1422 [30457:31878](+) psbD 1062 [29448:30509](+) psbE 258 [94376:94633](+) psbF 120 [94643:94762](+) psbH 228 [101517:101744](+) psbI 111 [8040:8150](+) psbJ 114 [95025:95138](+) psbK 186 [7257:7442](+) psbL 117 [94795:94911](+) psbM 111 [27481:27591](-) psbN 132 [101308:101439](-) psbT 108 [101109:101216](+) psbZ 189 [32421:32609](+) rbcL 1428 [117261:118688](-) rpl14 369 [107806:108174](-) rpl16 420 [109485:109493](-) [108277:108687](-) rpl2 831 [112288:112687](-) [111239:111669](-) rpl20 351 [91292:91642](+) rpl22 426 [110356:110781](-) rpl23 291 [112709:112999](-) rpl32 261 [52105:52365](-) rpl33 201 [92369:92569](-) rpl36 114 [106551:106664](-) rpoA 1011 [104843:105853](-) rpoB 3285 [22653:25937](-) rpoC1 2118 [22185:22626](-) [19767:21442](-) rpoC2 3522 [16075:19596](-) rps11 447 [105910:106356](-) rps12 381 [90457:90570](+) [66447:66678](+) [67218:67252](+) rps14 303 [33222:33524](-) rps15 261 [51396:51656](-) rps18 411 [91784:92194](-) rps19 294 [110886:111179](-) rps2 696 [15228:15923](-) rps3 681 [109585:110265](-) rps4 624 [41589:42212](-) rps7 468 [67297:67764](+) rps8 399 [107305:107703](-) ycf1 7203 [79545:86747](+) ycf2 7212 [70931:78142](+) ycf3 513 [40452:40580](-) [39513:39738](-) [38664:38821](-) ycf4 555 [98075:98629](-) 30 tRNA genes (29 are unique) Genes Location trnK-UUU [4001:4037](-) [1542:1576](-) trnQ-UUG [6869:6941](-) trnS-GCU [8219:8306](-) trnG-GCC [9058:9080](+) [9862:9909](+) trnR-UCU [10061:10132](+) trnC-GCA [26452:26522](+) trnD-GUC [27836:27909](-) trnY-GUA [28140:28223](-) trnE-UUC [28291:28363](-) trnT-GGU [28576:28647](+) trnS-UGA [32085:32170](-) trnG-UCC [32831:32901](+) trnfM-CAU [32994:33067](-) trnS-GGA [41295:41381](-) trnT-UGU [42510:42582](-) trnQ-UUG_copy2 [42736:42808](+) trnL-UAA [43019:43053](+) [43348:43397](+) trnF-GAA [43677:43749](+) trnN-GUU [55955:56026](-) trnR-ACG [56380:56453](-) trnA-UGC [60976:61013](-) [60169:60203](-) trnI-GAU [62006:62042](-) [61089:61123](-) trnV-GAC [65466:65537](-) trnI-CAU [70594:70666](+) trnL-UAG [87922:88001](-) trnP-UGG [93278:93351](+) trnW-CCA [93416:93489](+) trnM-CAU [121330:121402](-) trnV-UAC [121539:121576](+) [122108:122144](+) trnH-GUG [127889:127963](-) 0 rRNA genes (0 are unique) Genes Location This genome has 16 genes with intron(s). Details are as follows: 14 gene(s) have 1 intron(s) Genes Exons atpF [12970:13114](-) [11909:12318](-) rpoC1 [22185:22626](-) [19767:21442](-) ndhA [49542:50099](-) [48243:48791](-) ndhB [68019:68741](+) [69468:70223](+) petB [101888:101893](+) [102642:103283](+) petD [103444:103451](+) [104109:104598](+) rpl16 [109485:109493](-) [108277:108687](-) rpl2 [112288:112687](-) [111239:111669](-) trnK-UUU [4001:4037](-) [1542:1576](-) trnG-GCC [9058:9080](+) [9862:9909](+) trnL-UAA [43019:43053](+) [43348:43397](+) trnA-UGC [60976:61013](-) [60169:60203](-) trnI-GAU [62006:62042](-) [61089:61123](-) trnV-UAC [121539:121576](+) [122108:122144](+) 2 gene(s) have 2 intron(s) Genes Exons rps12 [90457:90570](+) [66447:66678](+) [67218:67252](+) ycf3 [40452:40580](-) [39513:39738](-) [38664:38821](-)