1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl2, rpl20, rpl22, rpl23, rpl23_copy2, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf2, ycf2_copy2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome rpl16, rpl32 Small subunit of ribosome rps15, rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf1, ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [8425:8574](-) [7271:7645](-) clpP (2/3) [57076:57410](-) [56226:56454](-) petB (2/2) [61157:61162](+) [61918:62559](+) petD (2/2) [62816:62824](+) [63543:64025](+) rpl2 (2/2) [71220:71613](-) [70138:70574](-) rpoC1 (2/2) [18046:18501](-) [15676:17292](-) rps12 (3/3) [55974:56087](-) [81657:81888](-) [81088:81113](-) rps12_copy2 (3/3) [55974:56087](-) [109833:110064](+) [110608:110633](+) ycf3 (3/3) [37414:37540](-) [36454:36683](-) [35530:35682](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon petD CTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 102 genes (89 unique genes). Including: 66 protein-coding genes (62 are unique) 28 tRNA genes (23 are unique) 8 rRNA genes (4 are unique) Details are as follows: 66 protein-coding genes (62 are unique) Genes Length Location accD 1470 [45818:47287](+) atpA 1530 [5656:7185](-) atpB 1473 [41617:43089](-) atpE 399 [41198:41596](-) atpF 525 [8425:8574](-) [7271:7645](-) atpH 246 [8867:9112](-) atpI 762 [9518:10279](-) ccsA 954 [93003:93956](+) cemA 600 [49121:49720](+) clpP 564 [57076:57410](-) [56226:56454](-) matK 1527 [1784:3310](-) petA 963 [49924:50886](+) petB 648 [61157:61162](+) [61918:62559](+) petD 492 [62816:62824](+) [63543:64025](+) petG 114 [52739:52852](+) petL 96 [52476:52571](+) petN 90 [22806:22895](+) psaA 2253 [32643:34895](-) psaB 2205 [30413:32617](-) psaC 246 [94208:94453](-) psaI 111 [47824:47934](+) psaJ 141 [53591:53731](+) psbA 1062 [202:1263](-) psbB 1527 [58718:60244](+) psbC 1422 [27186:28607](+) psbD 1062 [26177:27238](+) psbE 252 [51862:52113](-) psbF 120 [51733:51852](-) psbH 222 [60804:61025](+) psbI 111 [4657:4767](+) psbJ 123 [51317:51439](-) psbK 189 [4058:4246](+) psbL 147 [51590:51736](-) psbM 114 [23639:23752](-) psbN 132 [60574:60705](-) psbT 108 [60412:60519](+) psbZ 189 [29228:29416](+) rbcL 1428 [43809:45236](+) rpl14 369 [66712:67080](-) rpl2 831 [71220:71613](-) [70138:70574](-) rpl20 354 [54796:55149](-) rpl22 471 [69199:69669](-) rpl23 282 [71632:71913](-) rpl23_copy2 282 [119808:120089](+) rpl33 204 [53881:54084](+) rpl36 114 [65887:66000](-) rpoA 1008 [64223:65230](-) rpoB 3219 [18512:21730](-) rpoC1 2073 [18046:18501](-) [15676:17292](-) rpoC2 4182 [11307:15488](-) rps11 417 [65301:65717](-) rps12 372 [55974:56087](-) [81657:81888](-) [81088:81113](-) rps12_copy2 372 [55974:56087](-) [109833:110064](+) [110608:110633](+) rps14 303 [29968:30270](-) rps18 306 [54277:54582](+) rps19 225 [69755:69979](-) rps2 711 [10428:11138](-) rps3 657 [68558:69214](-) rps4 606 [38426:39031](-) rps7 468 [80565:81032](-) rps7_copy2 468 [110689:111156](+) rps8 408 [66150:66557](-) ycf2 6735 [72245:78979](+) ycf2_copy2 6735 [112742:119476](-) ycf3 510 [37414:37540](-) [36454:36683](-) [35530:35682](-) ycf4 561 [48296:48856](+) 28 tRNA genes (23 are unique) Genes Location trnQ-UUG [3668:3740](-) trnS-GCU [4846:4934](-) trnR-UCU [5369:5440](+) trnC-GCA [22494:22566](+) trnD-GUC [24164:24238](-) trnY-GUA [24622:24705](-) trnE-UUC [24753:24825](-) trnT-GGU [25272:25343](+) trnS-UGA [28835:28927](-) trnG-GCC [29631:29703](+) trnfM-CAU [29754:29827](-) trnS-GGA [38184:38270](+) trnT-UGU [39421:39493](-) trnF-GAA [40474:40546](+) trnM-CAU [40921:40995](+) trnW-CCA [52969:53048](-) trnP-UGG [53158:53231](-) trnI-CAU [72071:72144](-) trnL-CAA [79953:80033](-) trnV-GAC [83601:83672](+) trnR-ACG [90887:90960](+) trnN-GUU [91463:91535](-) trnL-UAG [92805:92884](+) trnN-GUU_copy2 [100186:100258](+) trnR-ACG_copy2 [100761:100834](-) trnV-GAC_copy2 [108049:108120](-) trnL-CAA_copy2 [111688:111768](+) trnI-CAU_copy2 [119577:119650](+) 8 rRNA genes (4 are unique) Genes Location rrn16S [83907:85397](-) rrn23S [87307:90118](-) rrn4.5S [90215:90316](-) rrn5S [90529:90659](+) rrn5S_copy2 [101062:101192](-) rrn4.5S_copy2 [101405:101506](+) rrn23S_copy2 [101603:104414](+) rrn16S_copy2 [106324:107814](+) This genome has 9 genes with intron(s). Details are as follows: 6 gene(s) have 1 intron(s) Genes Exons atpF [8425:8574](-) [7271:7645](-) rpoC1 [18046:18501](-) [15676:17292](-) clpP [57076:57410](-) [56226:56454](-) petB [61157:61162](+) [61918:62559](+) petD [62816:62824](+) [63543:64025](+) rpl2 [71220:71613](-) [70138:70574](-) 3 gene(s) have 2 intron(s) Genes Exons rps12 [55974:56087](-) [81657:81888](-) [81088:81113](-) ycf3 [37414:37540](-) [36454:36683](-) [35530:35682](-) rps12_copy2 [55974:56087](-) [109833:110064](+) [110608:110633](+)