1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl22_copy2, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps15, rps16, rps18, rps19, rps19_copy2, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf2, ycf2_copy2, ycf4 Other genes lhbA Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbZ Large subunit of ribosome None Small subunit of ribosome None DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [11858:12001](-) [10674:11084](-) clpP (3/3) [70574:70642](-) [69495:69785](-) [68580:68831](-) ndhA (2/2) [115438:115980](+) [117029:117559](+) ndhB (2/2) [95228:96004](-) [93772:94527](-) ndhB_copy2 (2/2) [140338:141114](+) [141815:142570](+) petB (2/2) [73488:73493](+) [74285:74926](+) petD (2/2) [75129:75134](+) [75904:76419](+) rpl16 (2/2) [81293:81301](-) [79875:80276](-) rpl2 (2/2) [84223:84627](-) [83131:83562](-) rpl2_copy2 (2/2) [151715:152119](+) [152780:153211](+) rpoC1 (2/2) [21803:22230](-) [19502:21113](-) rps12 (3/3) [68340:68453](-) [97418:97646](-) [96842:96867](-) rps12_copy2 (3/3) [68340:68453](-) [138696:138924](+) [139475:139500](+) rps16 (2/2) [5681:5720](-) [4602:4813](-) ycf3 (3/3) [42933:43064](-) [41981:42208](-) [41089:41247](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon cemA TTG rps19 GTG rpl2 ATA ndhD ATC rpl2_copy2 ATA rps19_copy2 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 125 genes (110 unique genes). Including: 87 protein-coding genes (79 are unique) 38 tRNA genes (31 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 16S ribosomal RNA [135092:136583](-) 23S ribosomal RNA [129879:132694](-) 4.5S ribosomal RNA [129678:129781](-) 5S ribosomal RNA [129347:129468](-) 87 protein-coding genes (79 are unique) Genes Length Location accD 1458 [55930:57387](+) atpA 1524 [9069:10592](-) atpB 1497 [51807:53303](-) atpE 405 [51406:51810](-) atpF 555 [11858:12001](-) [10674:11084](-) atpH 246 [12468:12713](-) atpI 744 [13283:14026](-) ccsA 972 [122025:122996](-) cemA 642 [60845:61486](-) clpP 612 [70574:70642](-) [69495:69785](-) [68580:68831](-) infA 234 [78462:78695](-) lhbA 189 [34568:34756](+) matK 1530 [1695:3224](-) ndhA 1074 [115438:115980](+) [117029:117559](+) ndhB 1533 [95228:96004](-) [93772:94527](-) ndhB_copy2 1533 [140338:141114](+) [141815:142570](+) ndhC 366 [48697:49062](-) ndhD 1506 [120297:121802](+) ndhE 306 [119266:119571](+) ndhF 2208 [124953:127160](+) ndhG 537 [118570:119106](+) ndhH 1182 [114255:115436](+) ndhI 534 [117659:118192](+) ndhJ 477 [47375:47851](-) ndhK 753 [47957:48709](-) petA 969 [59661:60629](-) petB 648 [73488:73493](+) [74285:74926](+) petD 522 [75129:75134](+) [75904:76419](+) petG 114 [64841:64954](+) petL 96 [64532:64627](+) petN 90 [27211:27300](+) psaA 2253 [38203:40455](-) psaB 2205 [35973:38177](-) psaC 246 [119933:120178](+) psaI 111 [58096:58206](+) psaJ 129 [65665:65793](+) psbA 1062 [105:1166](-) psbB 1527 [71045:72571](+) psbC 1422 [32590:34011](+) psbD 1062 [31581:32642](+) psbE 252 [62931:63182](-) psbF 120 [62801:62920](-) psbH 222 [73148:73369](+) psbI 111 [7128:7238](+) psbJ 123 [62414:62536](-) psbK 186 [6640:6825](+) psbL 117 [62662:62778](-) psbM 105 [28225:28329](-) psbN 132 [72905:73036](-) psbT 108 [72732:72839](+) rbcL 1443 [53907:55349](+) rpl14 369 [79382:79750](-) rpl16 411 [81293:81301](-) [79875:80276](-) rpl2 837 [84223:84627](-) [83131:83562](-) rpl20 366 [67227:67592](-) rpl22 309 [82183:82491](-) rpl22_copy2 309 [153851:154159](+) rpl23 282 [84634:84915](-) rpl23_copy2 282 [151427:151708](+) rpl2_copy2 837 [151715:152119](+) [152780:153211](+) rpl32 162 [123941:124102](-) rpl33 201 [66294:66494](+) rpl36 114 [78217:78330](-) rpoA 1026 [76574:77599](-) rpoB 3204 [22257:25460](-) rpoC1 2040 [21803:22230](-) [19502:21113](-) rpoC2 4038 [15297:19334](-) rps11 417 [77668:78084](-) rps12 369 [68340:68453](-) [97418:97646](-) [96842:96867](-) rps12_copy2 369 [68340:68453](-) [138696:138924](+) [139475:139500](+) rps14 303 [35533:35835](-) rps15 273 [113888:114160](+) rps16 252 [5681:5720](-) [4602:4813](-) rps18 306 [66662:66967](+) rps19 279 [82602:82880](-) rps19_copy2 279 [153462:153740](+) rps2 714 [14274:14987](-) rps3 654 [81458:82111](-) rps4 606 [44077:44682](-) rps7 468 [96316:96783](-) rps7_copy2 468 [139559:140026](+) rps8 399 [78812:79210](-) ycf1 5589 [107994:113582](+) ycf2 6897 [85223:92119](+) ycf2_copy2 6897 [144223:151119](-) ycf3 519 [42933:43064](-) [41981:42208](-) [41089:41247](-) ycf4 561 [58547:59107](+) 38 tRNA genes (31 are unique) Genes Location trnK-UUU [4009:4045](-) [1401:1435](-) trnQ-UUG [6242:6313](-) trnS-GCU [7384:7471](-) trnG-UCC [7987:8009](+) [8738:8785](+) trnR-UCU [8870:8941](+) trnC-GCA [26681:26751](+) trnD-GUC [29297:29370](-) trnY-GUA [29733:29816](-) trnE-UUC [29876:29948](-) trnT-GGU [30543:30614](+) trnS-UGA [34140:34232](-) trnG-GCC [35076:35146](+) trnfM-CAU [35300:35373](-) trnS-GGA [43693:43779](+) trnT-UGU [45011:45083](-) trnL-UAA [45939:45973](+) [46482:46531](+) trnF-GAA [46935:47007](+) trnV-UAC [50972:51010](-) [50352:50388](-) trnM-CAU [51176:51247](+) trnW-CCA [65067:65140](-) trnP-UGG [65304:65377](-) trnH-GUG [83013:83087](+) trnI-CAU [85081:85154](-) trnL-CAA [93132:93212](-) trnV-GAC [99460:99531](+) trnI-GAU [101552:101593](+) [102533:102567](+) trnA-UGC [102623:102660](+) [103463:103497](+) trnR-ACG [107244:107317](+) trnN-GUU [107598:107669](-) trnL-UAG [123077:123156](-) trnN [128673:128744](+) trnR-ACG_copy2 [129025:129098](-) trnA-UGC_copy2 [133682:133719](-) [132845:132879](-) trnI-GAU_copy2 [134749:134790](-) [133775:133809](-) trnV-GAC_copy2 [136811:136882](-) trnL-CAA_copy2 [143130:143210](+) trnI-CAU_copy2 [151188:151261](+) trnH-GUG_copy2 [153255:153329](-) 0 rRNA genes (0 are unique) Genes Location This genome has 23 genes with intron(s). Details are as follows: 19 gene(s) have 1 intron(s) Genes Exons rps16 [5681:5720](-) [4602:4813](-) atpF [11858:12001](-) [10674:11084](-) rpoC1 [21803:22230](-) [19502:21113](-) petB [73488:73493](+) [74285:74926](+) petD [75129:75134](+) [75904:76419](+) rpl16 [81293:81301](-) [79875:80276](-) rpl2 [84223:84627](-) [83131:83562](-) ndhB [95228:96004](-) [93772:94527](-) ndhA [115438:115980](+) [117029:117559](+) ndhB_copy2 [140338:141114](+) [141815:142570](+) rpl2_copy2 [151715:152119](+) [152780:153211](+) trnK-UUU [4009:4045](-) [1401:1435](-) trnG-UCC [7987:8009](+) [8738:8785](+) trnL-UAA [45939:45973](+) [46482:46531](+) trnV-UAC [50972:51010](-) [50352:50388](-) trnI-GAU [101552:101593](+) [102533:102567](+) trnA-UGC [102623:102660](+) [103463:103497](+) trnA-UGC_copy2 [133682:133719](-) [132845:132879](-) trnI-GAU_copy2 [134749:134790](-) [133775:133809](-) 4 gene(s) have 2 intron(s) Genes Exons rps12 [68340:68453](-) [97418:97646](-) [96842:96867](-) ycf3 [42933:43064](-) [41981:42208](-) [41089:41247](-) rps12_copy2 [68340:68453](-) [138696:138924](+) [139475:139500](+) clpP [70574:70642](-) [69495:69785](-) [68580:68831](-)