1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase None Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase None Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf1, ycf2, ycf2_copy2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex None Subunits of photosystem I psaI Subunits of photosystem II None Large subunit of ribosome rpl23, rpl32 Small subunit of ribosome rps16 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase matK Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (1/2) [5043:5585](-) clpP (3/3) [38466:38536](-) [37562:37853](-) [36734:36967](-) petB (2/2) [41175:41180](+) [41844:42485](+) petD (2/2) [42617:42624](+) [43285:43759](+) rpl16 (2/2) [47883:47891](-) [46633:47037](-) rpl2 (1/2) [49505:50317](-) rps12 (2/3) [36542:36655](-) [57307:57564](-) rps12_copy2 (2/3) [36542:36655](-) [78084:78341](+) ycf3 (1/3) [19592:20098](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbA GTG psbL ACG ycf2 ATC ycf2_copy2 ATC 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 97 genes (86 unique genes). Including: 61 protein-coding genes (58 are unique) 28 tRNA genes (24 are unique) 8 rRNA genes (4 are unique) Details are as follows: 61 protein-coding genes (58 are unique) Genes Length Location accD 1476 [27518:28993](+) atpA 1524 [3457:4980](-) atpB 1485 [23051:24535](-) atpE 258 [22797:23054](-) atpF 543 [5043:5585](-) atpH 246 [5783:6028](-) atpI 744 [6314:7057](-) ccsA 891 [69673:70563](-) cemA 699 [30160:30858](+) clpP 597 [38466:38536](-) [37562:37853](-) [36734:36967](-) petA 963 [31026:31988](+) petB 648 [41175:41180](+) [41844:42485](+) petD 483 [42617:42624](+) [43285:43759](+) petG 114 [33732:33845](+) petL 96 [33466:33561](+) petN 96 [9020:9115](+) psaA 2235 [17011:19245](-) psaB 2205 [14790:16994](-) psaC 246 [69263:69508](+) psaJ 135 [34346:34480](+) psbA 1062 [173:1234](-) psbB 1527 [38789:40315](+) psbC 1422 [11932:13353](+) psbD 1062 [10923:11984](+) psbE 255 [32946:33200](-) psbF 120 [32830:32949](-) psbH 210 [40848:41057](+) psbI 114 [2784:2897](+) psbJ 123 [32441:32563](-) psbK 180 [2331:2510](+) psbL 120 [32676:32795](-) psbM 105 [9463:9567](-) psbN 141 [40585:40725](-) psbT 108 [40423:40530](+) psbZ 189 [13679:13867](+) rbcL 1446 [25005:26450](+) rpl14 369 [46160:46528](-) rpl16 414 [47883:47891](-) [46633:47037](-) rpl2 813 [49505:50317](-) rpl20 363 [35460:35822](-) rpl22 444 [48612:49055](-) rpl33 201 [34592:34792](+) rpl36 114 [45268:45381](-) rps11 417 [44752:45168](-) rps12 372 [36542:36655](-) [57307:57564](-) rps12_copy2 372 [36542:36655](-) [78084:78341](+) rps14 303 [14392:14694](-) rps15 261 [68811:69071](+) rps18 450 [34914:35363](+) rps19 279 [49133:49411](-) rps2 711 [7135:7845](-) rps3 660 [47968:48627](-) rps4 594 [20474:21067](-) rps7 468 [56794:57261](-) rps7_copy2 468 [78387:78854](+) rps8 405 [45584:45988](-) ycf1 4959 [63638:68596](+) ycf2 5424 [50900:56323](+) ycf2_copy2 5424 [79325:84748](-) ycf3 507 [19592:20098](-) ycf4 531 [29336:29866](+) 28 tRNA genes (24 are unique) Genes Location trnH-GUG [1:69](-) trnQ-UUG [1907:1978](-) trnS-GCU [2933:3020](-) trnR-UCU [3289:3360](+) trnC-GCA [8578:8649](+) trnD-GUC [9662:9735](-) trnY-GUA [9790:9873](-) trnE-UUC [9942:10014](-) trnT-GGU [10215:10286](+) trnS-UGA [13458:13545](-) trnG-GCC [14035:14105](+) trnfM-CAU [14195:14268](-) trnS-GGA [20265:20354](+) trnT-UGU [21308:21380](-) trnL-UAA [21751:21785](+) [22042:22091](+) trnF-GAA [22214:22286](+) trnM-CAU [22555:22627](+) trnW-CCA [33909:33982](-) trnP-UGG [34147:34220](-) trnI-CAU [50486:50559](-) trnL-CAA [56410:56489](-) trnV-GAC [58148:58219](+) trnN-GUU [63453:63524](-) trnL-UAG [70684:70763](-) trnN-GUU_copy2 [72124:72195](+) trnV-GAC_copy2 [77429:77500](-) trnL-CAA_copy2 [79159:79238](+) trnI-CAU_copy2 [85089:85162](+) 8 rRNA genes (4 are unique) Genes Location rrn16 [58336:59827](+) rrn23 [60169:62998](+) rrn4.5 [63079:63181](+) rrn5 [63265:63385](+) rrn5_copy2 [72263:72383](-) rrn4.5_copy2 [72467:72569](-) rrn23_copy2 [72650:75479](-) rrn16_copy2 [75821:77312](-) This genome has 7 genes with intron(s). Details are as follows: 6 gene(s) have 1 intron(s) Genes Exons rps12 [36542:36655](-) [57307:57564](-) rps12_copy2 [36542:36655](-) [78084:78341](+) petB [41175:41180](+) [41844:42485](+) petD [42617:42624](+) [43285:43759](+) rpl16 [47883:47891](-) [46633:47037](-) trnL-UAA [21751:21785](+) [22042:22091](+) 1 gene(s) have 2 intron(s) Genes Exons clpP [38466:38536](-) [37562:37853](-) [36734:36967](-)