1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl23, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf1, ycf2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome rpl22 Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [14285:14428](-) [13185:13595](-) clpP (2/3) [40612:40974](+) [41584:41859](+) ndhA (2/2) [134132:134682](+) [135940:136474](+) ndhB (2/2) [104922:105644](+) [106338:107093](+) petB (2/2) [37788:37793](-) [36323:36964](-) petD (2/2) [36112:36119](-) [34920:35394](-) rpl16 (2/2) [58123:58131](+) [59242:59640](+) rpl2 (2/2) [54713:55103](+) [55817:56250](+) rpoC1 (2/2) [81795:82224](-) [79385:81009](-) rps12 (2/3) [2650:2763](+) [103772:104035](+) ycf3 (3/3) [64185:64310](+) [65039:65264](+) [66032:66186](+) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbL ACG petN ATT ndhD ATC 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 110 genes (109 unique genes). Including: 75 protein-coding genes (75 are unique) 31 tRNA genes (30 are unique) 4 rRNA genes (4 are unique) Details are as follows: 75 protein-coding genes (75 are unique) Genes Length Location atpA 1533 [11566:13098](-) atpB 1479 [50871:52349](+) atpE 402 [52372:52773](+) atpF 555 [14285:14428](-) [13185:13595](-) atpH 246 [14836:15081](-) atpI 744 [16048:16791](-) ccsA 972 [140875:141846](-) cemA 690 [69993:70682](+) clpP 639 [40612:40974](+) [41584:41859](+) matK 1521 [45785:47305](-) ndhA 1086 [134132:134682](+) [135940:136474](+) ndhB 1479 [104922:105644](+) [106338:107093](+) ndhC 363 [31994:32356](-) ndhD 1497 [139130:140626](+) ndhE 306 [138181:138486](+) ndhF 2235 [144233:146467](+) ndhG 531 [137426:137956](+) ndhH 1182 [132949:134130](+) ndhI 489 [136554:137042](+) ndhJ 477 [30655:31131](-) ndhK 684 [31268:31951](-) petA 963 [70894:71856](+) petB 648 [37788:37793](-) [36323:36964](-) petD 483 [36112:36119](-) [34920:35394](-) petG 114 [6725:6838](-) petL 96 [6977:7072](-) petN 96 [25116:25211](+) psaA 2253 [120537:122789](-) psaB 2205 [118307:120511](-) psaC 246 [138784:139029](+) psaI 105 [68530:68634](+) psaJ 129 [5594:5722](-) psbA 1062 [453:1514](-) psbB 1527 [38744:40270](-) psbC 1422 [114719:116140](+) psbD 1062 [113710:114771](+) psbE 252 [8049:8300](+) psbF 120 [8310:8429](+) psbH 222 [37926:38147](-) psbI 111 [20929:21039](-) psbJ 123 [8708:8830](+) psbK 186 [21469:21654](-) psbL 117 [8452:8568](+) psbM 105 [26117:26221](-) psbN 132 [38252:38383](+) psbT 108 [38449:38556](-) psbZ 189 [116759:116947](+) rbcL 1428 [48633:50060](-) rpl14 369 [59767:60135](+) rpl16 408 [58123:58131](+) [59242:59640](+) rpl2 825 [54713:55103](+) [55817:56250](+) rpl20 363 [3463:3825](+) rpl23 297 [54407:54703](+) rpl32 240 [143252:143491](-) rpl33 201 [4888:5088](-) rpl36 114 [61630:61743](+) rpoA 1002 [62583:63584](+) rpoB 3213 [82255:85467](-) rpoC1 2055 [81795:82224](-) [79385:81009](-) rpoC2 4176 [72931:77106](-) rps11 417 [62087:62503](+) rps12 378 [2650:2763](+) [103772:104035](+) rps14 303 [117881:118183](-) rps15 276 [132575:132850](+) rps18 492 [4071:4562](-) rps19 279 [56405:56683](+) rps2 696 [17023:17718](-) rps3 657 [57316:57972](+) rps4 603 [34109:34711](+) rps7 468 [104087:104554](+) rps8 405 [60784:61188](+) ycf1 5457 [87036:92492](-) ycf2 5610 [124143:129752](-) ycf3 507 [64185:64310](+) [65039:65264](+) [66032:66186](+) ycf4 675 [68875:69549](+) 31 tRNA genes (30 are unique) Genes Location trnH-GUG [3:77](-) trnP-UGG [6304:6377](+) trnW-CCA [6527:6600](+) trnG-UCC [10236:10258](+) [10958:11006](+) trnR-UCU [11313:11384](+) trnS-GCU [20685:20771](+) trnQ-UUG [22243:22314](+) trnC-GCA [23996:24066](+) trnD-GUC [26552:26625](-) trnY-GUA [27058:27141](-) trnE-UUC [27213:27285](-) trnL-UAA [29280:29316](+) [29831:29880](+) trnF-GAA [30012:30084](+) trnM-CAU [33482:33555](-) trnT-UGU [33746:33818](+) trnK-UUU [48040:48076](-) [45550:45584](-) trnM-CAU_copy2 [52924:52996](-) trnV-UAC [53174:53210](+) [53785:53821](+) trnS-GGA [63823:63910](-) trnN-GUU [93985:94056](+) trnR-ACG [94383:94456](-) trnA-UGC [99088:99125](-) [98234:98268](-) trnI-GAU [99909:99945](-) [99191:99225](-) trnV-GAC [101954:102025](-) trnL-CAA [107733:107813](-) trnT-GGU [112037:112109](+) trnS-UGA [116347:116435](-) trnG-GCC [117464:117534](+) trnfM-CAU [117650:117723](-) trnI-CAU [130723:130796](+) trnL-UAG [141986:142065](-) 4 rRNA genes (4 are unique) Genes Location rrn5 [94710:94830](-) rrn4.5 [95045:95149](-) rrn23 [95250:98059](-) rrn16 [100252:101744](-) This genome has 17 genes with intron(s). Details are as follows: 16 gene(s) have 1 intron(s) Genes Exons rps12 [2650:2763](+) [103772:104035](+) atpF [14285:14428](-) [13185:13595](-) petD [36112:36119](-) [34920:35394](-) petB [37788:37793](-) [36323:36964](-) clpP [40612:40974](+) [41584:41859](+) rpl2 [54713:55103](+) [55817:56250](+) rpl16 [58123:58131](+) [59242:59640](+) rpoC1 [81795:82224](-) [79385:81009](-) ndhB [104922:105644](+) [106338:107093](+) ndhA [134132:134682](+) [135940:136474](+) trnG-UCC [10236:10258](+) [10958:11006](+) trnL-UAA [29280:29316](+) [29831:29880](+) trnK-UUU [48040:48076](-) [45550:45584](-) trnV-UAC [53174:53210](+) [53785:53821](+) trnA-UGC [99088:99125](-) [98234:98268](-) trnI-GAU [99909:99945](-) [99191:99225](-) 1 gene(s) have 2 intron(s) Genes Exons ycf3 [64185:64310](+) [65039:65264](+) [66032:66186](+)