1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase ORF219, ORF470, atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ORF219, ORF470 Subunits of cytochrome b/f complex ORF219, ORF470, petA, petB, petD, petG, petL, petN Subunits of photosystem I ORF219, ORF470, psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II ORF219, ORF470, psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbN, psbT, ycf3 Large subunit of ribosome ORF219, ORF470, rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl32, rpl33, rpl36 Small subunit of ribosome ORF219, ORF470, rps11, rps12, rps14, rps16, rps18, rps19, rps2, rps3, rps4, rps7, rps8 DNA dependent RNA polymerase ORF219, ORF470, rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco ORF219, ORF470, rbcL c-type cytochrom synthesis gene ORF219, ORF470, ccsA Envelop membrane protein ORF219, ORF470 Maturase ORF219, ORF470 Protease ORF219, ORF470 Subunit of Acetyl-CoA-carboxylase ORF219, ORF470 Translational initiation factor ORF219, ORF470 Conserved open reading frames ORF219, ORF470, ycf12, ycf19, ycf33, ycf34, ycf35, ycf37, ycf39, ycf4, ycf41, ycf42, ycf46, ycf47, ycf54, ycf65, ycf66 Other genes ORF219, ORF470, acsF, atpD, atpG, cbbx, ccs1, chlB, chlI, chlL, chlN, clpC, dnaB, dnaK, ftrB, ftsH, groEL, ilvB, ilvH, petF, petJ, petM, psaD, psaE, psaF, psaL, psaM, psb28, psbV, psbX, psbY, rbcR, rbcS, rpl1, rpl11, rpl12, rpl13, rpl18, rpl19, rpl21, rpl24, rpl27, rpl29, rpl3, rpl31, rpl34, rpl35, rpl4, rpl5, rpl6, rpl9, rps1, rps10, rps13, rps17, rps20, rps5, rps9, secA, secY, sufB, sufC, tatC, thiG, thiS, tsf, tufA Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbM, psbZ Large subunit of ribosome None Small subunit of ribosome rps15 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf15, ycf2 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (1/2) [41340:41870](+) petB (1/2) [7560:8207](+) petD (1/2) [8321:8803](+) rpl16 (1/2) [119513:119923](-) rpl2 (1/2) [121349:122176](-) rpoC1 (1/2) [32288:34123](+) rps12 (1/3) [110846:111220](-) rps16 (1/2) [84931:85164](+) ycf3 (1/3) [61852:62361](+) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbF GTG rpl3 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 173 genes (166 unique genes). Including: 139 protein-coding genes (139 are unique) 28 tRNA genes (24 are unique) 6 rRNA genes (3 are unique) Details are as follows: 139 protein-coding genes (139 are unique) Genes Length Location ORF219 660 [24913:25572](+) ORF470 1413 [66306:67718](-) acsF 1113 [44334:45446](+) atpA 1506 [42597:44102](+) atpB 1437 [62558:63994](+) atpD 618 [41873:42490](+) atpE 405 [64010:64414](+) atpF 531 [41340:41870](+) atpG 465 [40788:41252](+) atpH 249 [40430:40678](+) atpI 747 [39617:40363](+) cbbx 906 [77993:78898](+) ccs1 1332 [23547:24878](+) ccsA 1032 [71360:72391](+) chlB 1533 [25906:27438](+) chlI 1023 [6154:7176](+) chlL 891 [70115:71005](-) chlN 1344 [68738:70081](-) clpC 2694 [58399:61092](-) dnaB 1740 [99908:101647](+) dnaK 1911 [15284:17194](+) ftrB 348 [77361:77708](+) ftsH 1887 [106434:108320](-) groEL 1587 [13031:14617](+) ilvB 2685 [86563:89247](-) ilvH 489 [46738:47226](+) petA 948 [65322:66269](+) petB 648 [7560:8207](+) petD 483 [8321:8803](+) petF 300 [103697:103996](-) petG 114 [55987:56100](-) petJ 333 [12529:12861](+) petL 96 [75234:75329](+) petM 99 [9056:9154](+) petN 90 [8914:9003](+) psaA 2253 [51012:53264](+) psaB 2205 [53286:55490](+) psaC 246 [57980:58225](+) psaD 399 [27799:28197](+) psaE 189 [108478:108666](+) psaF 552 [67999:68550](-) psaI 111 [77168:77278](-) psaJ 129 [67829:67957](-) psaL 438 [74622:75059](-) psaM 93 [5928:6020](+) psb28 357 [94493:94849](+) psbA 1083 [56576:57658](-) psbB 1533 [104185:105717](+) psbC 1425 [96418:97842](-) psbD 1056 [97790:98845](-) psbE 255 [76332:76586](+) psbF 132 [76635:76766](+) psbH 204 [106133:106336](+) psbI 126 [17307:17432](+) psbJ 120 [76967:77086](+) psbK 135 [56142:56276](-) psbL 117 [76778:76894](+) psbN 123 [105940:106062](-) psbT 126 [105751:105876](+) psbV 486 [11938:12423](+) psbX 132 [11234:11365](+) psbY 114 [73118:73231](-) rbcL 1467 [48379:49845](+) rbcR 930 [73604:74533](+) rbcS 420 [50009:50428](+) rpl1 690 [102445:103134](+) rpl11 426 [101983:102408](+) rpl12 399 [103165:103563](+) rpl13 435 [111975:112409](-) rpl14 366 [118647:119012](-) rpl16 411 [119513:119923](-) rpl18 330 [116509:116838](-) rpl19 399 [10159:10557](-) rpl2 828 [121349:122176](-) rpl20 345 [46314:46658](+) rpl21 318 [123893:124210](-) rpl22 348 [120655:121002](-) rpl23 303 [122207:122509](-) rpl24 249 [118398:118646](-) rpl27 249 [10772:11020](+) rpl29 192 [119317:119508](-) rpl3 642 [123154:123795](-) rpl31 234 [111252:111485](-) rpl32 195 [72732:72926](-) rpl33 240 [61332:61571](+) rpl34 147 [21823:21969](+) rpl35 195 [46100:46294](+) rpl36 114 [114459:114572](-) rpl4 642 [122502:123143](-) rpl5 564 [117817:118380](-) rpl6 537 [116845:117381](-) rpl9 468 [99428:99895](+) rpoA 1071 [112454:113524](-) rpoB 3372 [28879:32250](+) rpoC1 1836 [32288:34123](+) rpoC2 3825 [34152:37976](+) rps1 783 [94930:95712](+) rps10 321 [108726:109046](-) rps11 372 [113638:114009](-) rps12 375 [110846:111220](-) rps13 381 [114051:114431](-) rps14 303 [55571:55873](+) rps16 234 [84931:85164](+) rps17 255 [119037:119291](-) rps18 234 [61582:61815](+) rps19 285 [121021:121305](-) rps2 696 [38133:38828](+) rps20 291 [28574:28864](+) rps3 669 [119942:120610](-) rps4 627 [85919:86545](+) rps5 504 [115981:116484](-) rps7 471 [110357:110827](-) rps8 399 [117399:117797](-) rps9 429 [111530:111958](-) secA 2634 [19172:21805](+) secY 1293 [114615:115907](-) sufB 1446 [91863:93308](-) sufC 768 [91099:91866](-) tatC 789 [64449:65237](+) thiG 810 [17718:18527](+) thiS 237 [95709:95945](-) tsf 603 [38870:39472](+) tufA 1230 [109058:110287](-) ycf12 96 [77703:77798](-) ycf19 351 [84557:84907](+) ycf3 510 [61852:62361](+) ycf33 189 [89287:89475](-) ycf34 285 [47392:47676](-) ycf35 405 [93623:94027](-) ycf37 528 [5267:5794](-) ycf39 960 [89757:90716](+) ycf4 561 [75433:75993](+) ycf41 282 [90733:91014](+) ycf42 561 [45502:46062](+) ycf46 1476 [22038:23513](+) ycf47 222 [9228:9449](+) ycf54 342 [18718:19059](+) ycf65 345 [85182:85526](+) ycf66 315 [11547:11861](+) 28 tRNA genes (24 are unique) Genes Location trnA(tgc) [3122:3196](-) trnI(gat) [3198:3272](-) trnF(gaa) [7433:7505](+) trnM(cat) [10061:10148](+) trnD(gtc) [14651:14725](-) trnM(cat)_copy2 [14903:14975](+) trnS(gct) [14983:15071](+) trnG(gcc) [17560:17631](+) trnY(gta) [25622:25702](-) trnP(tgg) [27513:27587](+) trnM(cat)_copy3 [27652:27725](+) trnS(tga) [28370:28459](+) trnL(taa) [47973:48007](-) [47708:47758](-) trnC(gca) [48052:48124](-) trnE(ttc) [50723:50795](+) trnK(ttt) [58274:58347](+) trnL(tag) [72545:72628](-) trnG(tcc) [76110:76180](+) trnI(gat)_copy2 [80712:80786](+) trnA(tgc)_copy2 [80788:80862](+) trnQ(ttg) [85588:85661](-) trnR(acg) [85698:85771](-) trnR(tct) [94170:94243](-) trnV(tac) [94266:94337](-) trnH(gtg) [96011:96084](+) trnT(tgt) [96109:96182](+) trnN(gtt) [99059:99130](-) trnW(cca) [101796:101868](+) 6 rRNA genes (3 are unique) Genes Location rrn5 [5:120](-) rnl [145:3067](-) rns [3403:4881](-) rns_copy2 [79103:80581](+) rnl_copy2 [80917:83839](+) rrn5_copy2 [83864:83979](+) This genome has 1 genes with intron(s). Details are as follows: 1 gene(s) have 1 intron(s) Genes Exons trnL(taa) [47973:48007](-) [47708:47758](-)