1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhA_copy2, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhH_copy2, ndhI, ndhI_copy2, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps15, rps15_copy2, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf1, ycf1_copy2, ycf2, ycf2_copy2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome rpl22 Small subunit of ribosome rps16 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location rps12 402 join{[70349:70472](-), [98246:98525](-)} rps12_copy2 366 join{[70349:70472](-), [150633:150876](+)} 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [22168:22321](-) [21010:21434](-) ndhA (2/2) [117346:117898](+) [119196:119713](+) ndhA_copy2 (2/2) [131225:131777](-) [129410:129927](-) ndhB (2/2) [96062:96843](-) [94624:95401](-) ndhB_copy2 (2/2) [152280:153061](+) [153722:154499](+) petB (2/2) [74355:74360](+) [75146:75811](+) petD (2/2) [76024:76031](+) [76772:77210](+) rpl16 (2/2) [82414:82424](-) [80890:81280](-) rpl2 (2/2) [84915:85307](-) [83809:84258](-) rpl2_copy2 (2/2) [163816:164208](+) [164865:165314](+) rpoC1 (2/2) [33131:33562](-) [30747:32411](-) rps12 (2/3) [70350:70472](-) [98247:98525](-) rps12_copy2 (2/3) [70350:70472](-) [150634:150876](+) ycf3 (3/3) [55189:55312](-) [54207:54415](-) [53212:53358](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbL ACG rps19 GTG ndhD ACG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 123 genes (103 unique genes). Including: 85 protein-coding genes (74 are unique) 38 tRNA genes (29 are unique) 0 rRNA genes (0 are unique) Details are as follows: Nonstandard gene annotation ('/gene' tags possibly be missing in your GenBank file) 16S ribosomal RNA [147055:148546](-) 23S ribosomal RNA [141574:144402](-) 4.5S ribosomal RNA [141372:141476](-) 5S ribosomal RNA [141023:141144](-) 85 protein-coding genes (74 are unique) Genes Length Location atpA 1524 [19424:20947](-) atpB 1470 [7487:8956](+) atpE 402 [8980:9381](+) atpF 579 [22168:22321](-) [21010:21434](-) atpH 246 [22765:23010](-) atpI 744 [24403:25146](-) ccsA 960 [124075:125034](+) cemA 699 [61077:61775](-) matK 1524 [2084:3607](-) ndhA 1071 [117346:117898](+) [119196:119713](+) ndhA_copy2 1071 [131225:131777](-) [129410:129927](-) ndhB 1560 [96062:96843](-) [94624:95401](-) ndhB_copy2 1560 [152280:153061](+) [153722:154499](+) ndhC 363 [11171:11533](+) ndhD 1500 [125345:126844](-) ndhE 306 [127496:127801](-) ndhF 2238 [120561:122798](-) ndhG 531 [127993:128523](-) ndhH 1182 [116163:117344](+) ndhH_copy2 1182 [131779:132960](-) ndhI 498 [119792:120289](+) ndhI_copy2 498 [128834:129331](-) ndhJ 477 [12393:12869](+) ndhK 690 [11579:12268](+) petA 963 [59899:60861](-) petB 672 [74355:74360](+) [75146:75811](+) petD 447 [76024:76031](+) [76772:77210](+) petG 114 [66671:66784](+) petL 96 [66387:66482](+) petN 90 [38573:38662](+) psaA 2253 [50224:52476](-) psaB 2205 [47994:50198](-) psaC 246 [126973:127218](-) psaI 114 [63101:63214](-) psaJ 138 [67484:67621](+) psbA 1062 [420:1481](-) psbB 1527 [71879:73405](+) psbC 1422 [44363:45784](+) psbD 1062 [43354:44415](+) psbE 252 [58070:58321](+) psbF 120 [58331:58450](+) psbH 228 [74008:74235](+) psbI 111 [16567:16677](+) psbJ 123 [58743:58865](+) psbK 186 [15972:16157](+) psbL 117 [58478:58594](+) psbM 105 [39724:39828](-) psbN 132 [73747:73878](-) psbT 108 [73574:73681](+) psbZ 189 [46509:46697](+) rbcL 1428 [5264:6691](-) rpl14 369 [80386:80754](-) rpl16 402 [82414:82424](-) [80890:81280](-) rpl2 843 [84915:85307](-) [83809:84258](-) rpl20 354 [69127:69480](-) rpl23 282 [85326:85607](-) rpl23_copy2 282 [163516:163797](+) rpl2_copy2 843 [163816:164208](+) [164865:165314](+) rpl32 168 [123249:123416](+) rpl33 201 [68109:68309](+) rpl36 114 [79159:79272](-) rpoA 996 [77402:78397](-) rpoB 3216 [33592:36807](-) rpoC1 2097 [33131:33562](-) [30747:32411](-) rpoC2 4176 [26391:30566](-) rps11 438 [78470:78907](-) rps12 402 [70350:70472](-) [98247:98525](-) rps12_copy2 366 [70350:70472](-) [150634:150876](+) rps14 303 [47560:47862](-) rps15 273 [115781:116053](+) rps15_copy2 273 [133070:133342](-) rps18 375 [68521:68895](+) rps19 288 [83412:83699](-) rps2 705 [25382:26086](-) rps3 651 [82527:83177](-) rps4 606 [55967:56572](-) rps7 471 [97156:97626](-) rps7_copy2 471 [151497:151967](+) rps8 405 [79783:80187](-) ycf1 5496 [109909:115404](+) ycf1_copy2 5496 [133719:139214](-) ycf2 6642 [86437:93078](+) ycf2_copy2 6642 [156045:162686](-) ycf3 480 [55189:55312](-) [54207:54415](-) [53212:53358](-) ycf4 555 [62368:62922](-) 38 tRNA genes (29 are unique) Genes Location trnH-GUG [78:152](-) trnK-UUU [4349:4385](-) [1750:1778](-) trnM-CAU [9702:9774](-) trnV-UAC [9953:9991](+) [10619:10655](+) trnF-GAA [13503:13575](-) trnL-UAA [14545:14581](-) [13970:14019](-) trnQ-UUG [15269:15340](-) trnS-GCU [16826:16919](-) trnR-UCU [18919:18990](+) trnC-GCA [37859:37939](+) trnD-GUC [40286:40359](-) trnY-GUA [40857:40940](-) trnE-UUC [41007:41079](-) trnT-GGU [41756:41827](+) trnS-UGA [46033:46125](-) trnG-UCC [47176:47246](+) trnfM-CAU [47318:47391](-) trnS-GGA [55603:55689](+) trnT-UGU [56930:57002](-) trnW-CCA [66900:66973](-) trnP-UGG [67118:67191](-) trnQ-UUG_copy2 [86005:86076](-) trnI-CAU [86250:86323](-) trnL-CAA [93964:94044](-) trnV-GAC [100263:100334](+) trnI-GAU [102608:102649](+) [103583:103617](+) trnA-UGC [103682:103719](+) [104528:104562](+) trnR-ACG [108370:108443](+) trnN-GUU [109526:109597](-) trnL-UAG [123922:124001](+) trnN-GUU_copy2 [139526:139597](+) trnR-ACG_copy2 [140680:140753](-) trnA-UGC_copy2 [145404:145441](-) [144561:144595](-) trnI-GAU_copy2 [146474:146515](-) [145506:145540](-) trnV-GAC_copy2 [148789:148860](-) trnL-CAA_copy2 [155079:155159](+) trnI-CAU_copy2 [162800:162873](+) trnQ-UUG_copy3 [163047:163118](+) 0 rRNA genes (0 are unique) Genes Location This genome has 21 genes with intron(s). Details are as follows: 20 gene(s) have 1 intron(s) Genes Exons rps12 [70350:70472](-) [98247:98525](-) atpF [22168:22321](-) [21010:21434](-) rpoC1 [33131:33562](-) [30747:32411](-) rps12_copy2 [70350:70472](-) [150634:150876](+) petB [74355:74360](+) [75146:75811](+) petD [76024:76031](+) [76772:77210](+) rpl16 [82414:82424](-) [80890:81280](-) rpl2 [84915:85307](-) [83809:84258](-) ndhB [96062:96843](-) [94624:95401](-) ndhA [117346:117898](+) [119196:119713](+) ndhA_copy2 [131225:131777](-) [129410:129927](-) ndhB_copy2 [152280:153061](+) [153722:154499](+) rpl2_copy2 [163816:164208](+) [164865:165314](+) trnK-UUU [4349:4385](-) [1750:1778](-) trnV-UAC [9953:9991](+) [10619:10655](+) trnL-UAA [14545:14581](-) [13970:14019](-) trnI-GAU [102608:102649](+) [103583:103617](+) trnA-UGC [103682:103719](+) [104528:104562](+) trnA-UGC_copy2 [145404:145441](-) [144561:144595](-) trnI-GAU_copy2 [146474:146515](-) [145506:145540](-) 1 gene(s) have 2 intron(s) Genes Exons ycf3 [55189:55312](-) [54207:54415](-) [53212:53358](-)