1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps15, rps16, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor None Conserved open reading frames ycf1, ycf2, ycf2_copy2, ycf4 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbJ Large subunit of ribosome None Small subunit of ribosome None DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase None Translational initiation factor infA Conserved open reading frames ycf15 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [12325:12469](-) [11196:11605](-) clpP (3/3) [71446:71516](-) [70268:70559](-) [69465:69692](-) ndhA (2/2) [121570:122122](-) [119955:120484](-) ndhB (2/2) [95983:96705](-) [94536:95297](-) ndhB_copy2 (2/2) [141762:142484](+) [143170:143931](+) petB (2/2) [74476:74481](+) [75281:75922](+) petD (2/2) [76124:76131](+) [76856:77330](+) rpl16 (2/2) [82242:82250](-) [80751:81149](-) rpl2 (2/2) [85071:85460](-) [83954:84388](-) rpl2_copy2 (2/2) [153007:153396](+) [154079:154513](+) rpoC1 (2/2) [22324:22755](-) [19931:21541](-) rps12 (3/3) [69167:69280](-) [98161:98392](-) [97598:97623](-) rps12_copy2 (3/3) [69167:69280](-) [140075:140306](+) [140844:140869](+) rps16 (2/2) [5884:5923](-) [4780:5006](-) ycf3 (3/3) [43893:44018](-) [42953:43180](-) [42009:42161](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon rps19 GTG ndhD ATT ycf1 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 128 genes (111 unique genes). Including: 83 protein-coding genes (77 are unique) 37 tRNA genes (30 are unique) 8 rRNA genes (4 are unique) Details are as follows: 83 protein-coding genes (77 are unique) Genes Length Location accD 1455 [56626:58080](+) atpA 1524 [9607:11130](-) atpB 1509 [52205:53713](-) atpE 399 [51810:52208](-) atpF 555 [12325:12469](-) [11196:11605](-) atpH 246 [12945:13190](-) atpI 750 [13693:14442](-) ccsA 987 [114610:115596](+) cemA 690 [60246:60935](+) clpP 591 [71446:71516](-) [70268:70559](-) [69465:69692](-) matK 1581 [1926:3506](-) ndhA 1083 [121570:122122](-) [119955:120484](-) ndhB 1485 [95983:96705](-) [94536:95297](-) ndhB_copy2 1485 [141762:142484](+) [143170:143931](+) ndhC 363 [49547:49909](-) ndhD 1575 [115809:117383](-) ndhE 306 [117955:118260](-) ndhF 2241 [110191:112431](-) ndhG 531 [118507:119037](-) ndhH 1182 [122124:123305](-) ndhI 504 [119367:119870](-) ndhJ 477 [48227:48703](-) ndhK 678 [48806:49483](-) petA 963 [61161:62123](+) petB 648 [74476:74481](+) [75281:75922](+) petD 483 [76124:76131](+) [76856:77330](+) petG 114 [65523:65636](+) petL 96 [65240:65335](+) petN 90 [27687:27776](+) psaA 2253 [39028:41280](-) psaB 2205 [36798:39002](-) psaC 246 [117468:117713](-) psaI 114 [58763:58876](+) psaJ 129 [66457:66585](+) psbA 1062 [261:1322](-) psbB 1527 [72017:73543](+) psbC 1422 [33197:34618](+) psbD 1062 [32188:33249](+) psbE 252 [63598:63849](-) psbF 120 [63469:63588](-) psbH 222 [74124:74345](+) psbI 111 [7373:7483](+) psbK 186 [6777:6962](+) psbL 117 [63331:63447](-) psbM 105 [28289:28393](-) psbN 132 [73888:74019](-) psbT 102 [73721:73822](+) psbZ 189 [35232:35420](+) rbcL 1440 [54502:55941](+) rpl14 369 [80259:80627](-) rpl16 408 [82242:82250](-) [80751:81149](-) rpl2 825 [85071:85460](-) [83954:84388](-) rpl20 354 [68050:68403](-) rpl22 483 [83082:83564](-) rpl23 282 [85479:85760](-) rpl23_copy2 282 [152707:152988](+) rpl2_copy2 825 [153007:153396](+) [154079:154513](+) rpl32 159 [113284:113442](+) rpl33 201 [67023:67223](+) rpl36 114 [79106:79219](-) rpoA 984 [77530:78513](-) rpoB 3219 [22782:26000](-) rpoC1 2043 [22324:22755](-) [19931:21541](-) rpoC2 4122 [15628:19749](-) rps11 417 [78578:78994](-) rps12 372 [69167:69280](-) [98161:98392](-) [97598:97623](-) rps12_copy2 372 [69167:69280](-) [140075:140306](+) [140844:140869](+) rps14 303 [36363:36665](-) rps15 267 [123398:123664](-) rps16 267 [5884:5923](-) [4780:5006](-) rps18 306 [67454:67759](+) rps19 279 [83620:83898](-) rps2 711 [14681:15391](-) rps3 657 [82441:83097](-) rps4 606 [44650:45255](-) rps7 468 [97077:97544](-) rps7_copy2 468 [140923:141390](+) rps8 405 [79673:80077](-) ycf1 5337 [123942:129278](-) ycf2 6870 [86091:92960](+) ycf2_copy2 6870 [145507:152376](-) ycf3 507 [43893:44018](-) [42953:43180](-) [42009:42161](-) ycf4 555 [59262:59816](+) 37 tRNA genes (30 are unique) Genes Location trnH-GUG [5:77](-) trnK-UUU [4178:4214](-) [1595:1629](-) trnQ-UUG [6366:6437](-) trnS-GCU [7575:7662](+) trnG-GCC [8302:8324](+) [9044:9092](+) trnR-UCU [9246:9317](+) trnC-GCA [26961:27039](+) trnD-GUC [29418:29491](-) trnY-GUA [29936:30019](-) trnE-UUC [30079:30151](-) trnT-GGU [30923:30994](+) trnS-UGA [34796:34880](-) trnG-UCC [35919:35989](+) trnfM-CAU [36130:36203](-) trnS-GGA [44255:44341](+) trnT-UGU [45640:45712](-) trnL-UAA [46512:46548](+) [47061:47110](+) trnF-GAA [47627:47699](+) trnV-UAC [51373:51411](-) [50735:50771](-) trnM-CAU [51597:51669](+) trnW-CCA [65766:65839](-) trnP-UGG [66027:66100](-) trnI-CAU [85929:86002](-) trnL-CAA [93877:93957](-) trnV-GAC [100304:100375](+) trnI-GAU [102396:102437](+) [103380:103414](+) trnA-UGC [103479:103516](+) [104317:104351](+) trnR-ACG [108129:108202](+) trnN-GUU [108797:108868](-) trnL-UAG [114398:114477](+) trnN-GUU_copy2 [129599:129670](+) trnR-ACG_copy2 [130265:130338](-) trnA-UGC_copy2 [134951:134988](-) [134116:134150](-) trnI-GAU_copy2 [136030:136071](-) [135053:135087](-) trnV-GAC_copy2 [138092:138163](-) trnL-CAA_copy2 [144510:144590](+) trnI-CAU_copy2 [152465:152538](+) 8 rRNA genes (4 are unique) Genes Location rrn16 [100607:102097](+) rrn23 [104504:107313](+) rrn4.5 [107412:107514](+) rrn5 [107762:107882](+) rrn5_copy2 [130585:130705](-) rrn4.5_copy2 [130953:131055](-) rrn23_copy2 [131154:133963](-) rrn16_copy2 [136370:137860](-) This genome has 23 genes with intron(s). Details are as follows: 19 gene(s) have 1 intron(s) Genes Exons rps16 [5884:5923](-) [4780:5006](-) atpF [12325:12469](-) [11196:11605](-) rpoC1 [22324:22755](-) [19931:21541](-) petB [74476:74481](+) [75281:75922](+) petD [76124:76131](+) [76856:77330](+) rpl16 [82242:82250](-) [80751:81149](-) rpl2 [85071:85460](-) [83954:84388](-) ndhB [95983:96705](-) [94536:95297](-) ndhA [121570:122122](-) [119955:120484](-) ndhB_copy2 [141762:142484](+) [143170:143931](+) rpl2_copy2 [153007:153396](+) [154079:154513](+) trnK-UUU [4178:4214](-) [1595:1629](-) trnG-GCC [8302:8324](+) [9044:9092](+) trnL-UAA [46512:46548](+) [47061:47110](+) trnV-UAC [51373:51411](-) [50735:50771](-) trnI-GAU [102396:102437](+) [103380:103414](+) trnA-UGC [103479:103516](+) [104317:104351](+) trnA-UGC_copy2 [134951:134988](-) [134116:134150](-) trnI-GAU_copy2 [136030:136071](-) [135053:135087](-) 4 gene(s) have 2 intron(s) Genes Exons rps12 [69167:69280](-) [98161:98392](-) [97598:97623](-) ycf3 [43893:44018](-) [42953:43180](-) [42009:42161](-) rps12_copy2 [69167:69280](-) [140075:140306](+) [140844:140869](+) clpP [71446:71516](-) [70268:70559](-) [69465:69692](-)