1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbM, psbT, psbZ Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps14, rps15, rps16, rps18, rps19, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf1_copy2, ycf2, ycf2_copy2 Other genes clpP1, pafI, pafII, pbf1 Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II psbL, psbN, ycf3 Large subunit of ribosome None Small subunit of ribosome None DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf15, ycf4 Other genes None 2. Multicopy genes with different lengths: Genes Length Location ycf1 1083 [113113:114196](+) ycf1_copy2 5634 [128845:134479](-) 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [13412:13556](-) [12275:12684](-) ndhA (2/2) [126328:126880](-) [124640:125178](-) ndhB (2/2) [100040:100816](-) [98615:99370](-) ndhB_copy2 (2/2) [146777:147553](+) [148223:148978](+) petB (2/2) [78384:78389](+) [79187:79828](+) petD (2/2) [80019:80026](+) [80751:81225](+) rpl16 (2/2) [86200:86208](-) [84794:85192](-) rpl2 (2/2) [89102:89492](-) [87982:88415](-) rpl2_copy2 (2/2) [158101:158491](+) [159178:159611](+) rpoC1 (2/2) [24043:24474](-) [21688:23298](-) rps12 (3/3) [73119:73232](-) [102225:102456](-) [101658:101683](-) rps16 (2/2) [6371:6410](-) [5248:5477](-) ### The exon column shows the number of (actual exons/expected exons). ### Uncommon intron-contained genes: pafI 3 [47071:47194](-) [46133:46362](-) [45235:45387](-)] clpP1 3 [75377:75447](-) [74269:74560](-) [73410:73637](-)] 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon psbC GTG infA ATT rps19 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 129 genes (112 unique genes). Including: 84 protein-coding genes (78 are unique) 37 tRNA genes (30 are unique) 8 rRNA genes (4 are unique) Details are as follows: 84 protein-coding genes (78 are unique) Genes Length Location accD 1476 [60423:61898](+) atpA 1524 [10698:12221](-) atpB 1479 [56137:57615](-) atpE 402 [55713:56114](-) atpF 555 [13412:13556](-) [12275:12684](-) atpH 246 [14008:14253](-) atpI 744 [15413:16156](-) ccsA 966 [119253:120218](+) cemA 690 [64305:64994](+) clpP1 591 [75377:75447](-) [74269:74560](-) [73410:73637](-) infA 90 [83348:83437](-) matK 1521 [2025:3545](-) ndhA 1092 [126328:126880](-) [124640:125178](-) ndhB 1533 [100040:100816](-) [98615:99370](-) ndhB_copy2 1533 [146777:147553](+) [148223:148978](+) ndhC 363 [53613:53975](-) ndhD 1503 [120482:121984](-) ndhE 306 [122600:122905](-) ndhF 2265 [114176:116440](-) ndhG 531 [123140:123670](-) ndhH 1182 [126882:128063](-) ndhI 501 [124060:124560](-) ndhJ 477 [52269:52745](-) ndhK 681 [52883:53563](-) pafI 507 [47071:47194](-) [46133:46362](-) [45235:45387](-) pafII 555 [63229:63783](+) pbf1 132 [77787:77918](-) petA 963 [65210:66172](+) petB 648 [78384:78389](+) [79187:79828](+) petD 483 [80019:80026](+) [80751:81225](+) petG 114 [69427:69540](+) petL 96 [69173:69268](+) petN 90 [29918:30007](+) psaA 2253 [42284:44536](-) psaB 2205 [40054:42258](-) psaC 246 [122109:122354](-) psaI 114 [62711:62824](+) psaJ 135 [70399:70533](+) psbA 1062 [368:1429](-) psbB 1527 [75908:77434](+) psbC 1386 [36422:37807](+) psbD 1062 [35377:36438](+) psbE 252 [67680:67931](-) psbF 120 [67551:67670](-) psbH 225 [78037:78261](+) psbI 111 [8143:8253](+) psbJ 123 [67163:67285](-) psbK 186 [7698:7883](+) psbM 105 [31220:31324](-) psbT 108 [77619:77726](+) psbZ 189 [38465:38653](+) rbcL 1428 [58381:59808](+) rpl14 369 [84273:84641](-) rpl16 408 [86200:86208](-) [84794:85192](-) rpl2 825 [89102:89492](-) [87982:88415](-) rpl20 354 [71999:72352](-) rpl22 426 [87146:87571](-) rpl23 282 [89511:89792](-) rpl23_copy2 282 [157801:158082](+) rpl2_copy2 825 [158101:158491](+) [159178:159611](+) rpl32 159 [117502:117660](+) rpl33 201 [71003:71203](+) rpl36 114 [83089:83202](-) rpoA 996 [81452:82447](-) rpoB 3207 [24506:27712](-) rpoC1 2043 [24043:24474](-) [21688:23298](-) rpoC2 4122 [17389:21510](-) rps11 417 [82512:82928](-) rps12 372 [73119:73232](-) [102225:102456](-) [101658:101683](-) rps14 303 [39620:39922](-) rps15 273 [128174:128446](-) rps16 270 [6371:6410](-) [5248:5477](-) rps18 306 [71420:71725](+) rps19 279 [87633:87911](-) rps2 711 [16378:17088](-) rps3 657 [86360:87016](-) rps4 606 [48190:48795](-) rps7 468 [101138:101605](-) rps7_copy2 468 [145988:146455](+) rps8 405 [83662:84066](-) ycf1 1083 [113114:114196](+) ycf1_copy2 5634 [128846:134479](-) ycf2 6840 [90120:96959](+) ycf2_copy2 6840 [150634:157473](-) 37 tRNA genes (30 are unique) Genes Location trnH-GUG [27:101](-) trnK-UUU [4250:4286](-) [1719:1753](-) trnQ-UUG [7231:7302](-) trnS-GCU [8390:8477](-) trnG-UCC [9114:9136](+) [9836:9883](+) trnR-UCU [10069:10140](+) trnC-GCA [28917:28987](+) trnD-GUC [32552:32625](-) trnY-GUA [33069:33152](-) trnE-UUC [33212:33284](-) trnT-GGU [33820:33891](+) trnS-UGA [38062:38154](-) trnG-GCC [39161:39231](+) trnfM-CAU [39396:39469](-) trnS-GGA [47804:47890](+) trnT-UGU [49204:49276](-) trnL-UAA [50487:50521](+) [51044:51093](+) trnF-GAA [51503:51575](+) trnV-UAC [55201:55239](-) [54572:54606](-) trnM-CAU [55407:55479](+) trnW-CCA [69666:69739](-) trnP-UGG [69918:69991](-) trnI-CAU [89958:90031](-) trnL-CAA [97911:97991](-) trnV-GAC [104182:104253](+) trnI-GAU [106268:106304](+) [107253:107287](+) trnA-UGC [107352:107389](+) [108197:108231](+) trnR-ACG [112032:112105](+) trnN-GUU [112715:112786](-) trnL-UAG [119079:119158](+) trnN-GUU_copy2 [134807:134878](+) trnR-ACG_copy2 [135488:135561](-) trnA-UGC_copy2 [140204:140241](-) [139362:139396](-) trnI-GAU_copy2 [141289:141325](-) [140306:140340](-) trnV-GAC_copy2 [143340:143411](-) trnL-CAA_copy2 [149602:149682](+) trnI-CAU_copy2 [157562:157635](+) 8 rRNA genes (4 are unique) Genes Location rrn16 [104481:105971](+) rrn23 [108395:111203](+) rrn4.5 [111302:111404](+) rrn5 [111658:111778](+) rrn5_copy2 [135815:135935](-) rrn4.5_copy2 [136189:136291](-) rrn23_copy2 [136390:139198](-) rrn16_copy2 [141622:143112](-) This genome has 22 genes with intron(s). Details are as follows: 19 gene(s) have 1 intron(s) Genes Exons rps16 [6371:6410](-) [5248:5477](-) atpF [13412:13556](-) [12275:12684](-) rpoC1 [24043:24474](-) [21688:23298](-) petB [78384:78389](+) [79187:79828](+) petD [80019:80026](+) [80751:81225](+) rpl16 [86200:86208](-) [84794:85192](-) rpl2 [89102:89492](-) [87982:88415](-) ndhB [100040:100816](-) [98615:99370](-) ndhA [126328:126880](-) [124640:125178](-) ndhB_copy2 [146777:147553](+) [148223:148978](+) rpl2_copy2 [158101:158491](+) [159178:159611](+) trnK-UUU [4250:4286](-) [1719:1753](-) trnG-UCC [9114:9136](+) [9836:9883](+) trnL-UAA [50487:50521](+) [51044:51093](+) trnV-UAC [55201:55239](-) [54572:54606](-) trnI-GAU [106268:106304](+) [107253:107287](+) trnA-UGC [107352:107389](+) [108197:108231](+) trnA-UGC_copy2 [140204:140241](-) [139362:139396](-) trnI-GAU_copy2 [141289:141325](-) [140306:140340](-) 3 gene(s) have 2 intron(s) Genes Exons rps12 [73119:73232](-) [102225:102456](-) [101658:101683](-) pafI [47071:47194](-) [46133:46362](-) [45235:45387](-) clpP1 [75377:75447](-) [74269:74560](-) [73410:73637](-)