1. Annotated protein coding genes: Group of genes Name of genes Subunits of ATP synthase atpA, atpB, atpE, atpF, atpH, atpI Subunits of NADH-dehydrogenase ndhA, ndhB, ndhB_copy2, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK Subunits of cytochrome b/f complex petA, petB, petD, petG, petL, petN Subunits of photosystem I psaA, psaB, psaC, psaI, psaJ Subunits of photosystem II psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ, ycf3 Large subunit of ribosome rpl14, rpl16, rpl2, rpl20, rpl22, rpl23, rpl23_copy2, rpl2_copy2, rpl32, rpl33, rpl36 Small subunit of ribosome rps11, rps12, rps12_copy2, rps14, rps16, rps18, rps19, rps19_copy2, rps2, rps3, rps4, rps7, rps7_copy2, rps8 DNA dependent RNA polymerase rpoA, rpoB, rpoC1, rpoC2 Subunit of rubisco rbcL c-type cytochrom synthesis gene ccsA Envelop membrane protein cemA Maturase matK Protease clpP Subunit of Acetyl-CoA-carboxylase None Translational initiation factor None Conserved open reading frames ycf4, ycf68, ycf68_copy2 Other genes None Possible missing protein coding genes: Group of genes Name of genes Subunits of ATP synthase None Subunits of NADH-dehydrogenase None Subunits of cytochrome b/f complex None Subunits of photosystem I None Subunits of photosystem II None Large subunit of ribosome None Small subunit of ribosome rps15 DNA dependent RNA polymerase None Subunit of rubisco None c-type cytochrom synthesis gene None Envelop membrane protein None Maturase None Protease None Subunit of Acetyl-CoA-carboxylase accD Translational initiation factor infA Conserved open reading frames ycf1, ycf15, ycf2 Other genes None 2. Multicopy genes with different lengths: Genes Length Location None None None 3. Common intron-contained genes: Genes exons exon1 exon2 exon3 atpF (2/2) [34806:34964](+) [35799:36206](+) clpP (1/3) [69902:70552](-) ndhA (2/2) [116022:116570](-) [114466:115005](-) ndhB (2/2) [90094:90870](-) [88626:89381](-) ndhB_copy2 (2/2) [131357:132133](+) [132846:133601](+) petB (2/2) [73499:73504](+) [74329:74970](+) petD (2/2) [75159:75166](+) [75915:76389](+) rpl16 (2/2) [81468:81476](-) [79975:80382](-) rpl2 (2/2) [84552:84956](-) [83460:83891](-) rpl2_copy2 (2/2) [137271:137675](+) [138336:138767](+) rpoC1 (1/2) [24524:26572](+) rps12 (2/3) [69648:69761](-) [92257:92499](-) rps12_copy2 (2/3) [69648:69761](-) [129728:129970](+) rps16 (2/2) [5567:5606](-) [4503:4720](-) ycf3 (3/3) [45814:45945](-) [44854:45081](-) [43970:44128](-) ### The exon column shows the number of (actual exons/expected exons). ### 4. Genes with internal stop codon: None 5. Genes with nonstandard start codon: Genes Start codon rps19 GTG rpl2 ATA rpl2_copy2 ATA rps19_copy2 GTG 6. Genes with nonstandard stop codon: Genes Stop codon None None 7. Conclusion This genome has 129 genes (108 unique genes). Including: 82 protein-coding genes (75 are unique) 39 tRNA genes (29 are unique) 8 rRNA genes (4 are unique) Details are as follows: 82 protein-coding genes (75 are unique) Genes Length Location atpA 1524 [36299:37822](+) atpB 1497 [54370:55866](-) atpE 414 [53960:54373](-) atpF 567 [34806:34964](+) [35799:36206](+) atpH 246 [34104:34349](+) atpI 744 [32542:33285](+) ccsA 978 [108985:109962](+) cemA 693 [60803:61495](+) clpP 651 [69902:70552](-) matK 1536 [1680:3215](-) ndhA 1089 [116022:116570](-) [114466:115005](-) ndhB 1533 [90094:90870](-) [88626:89381](-) ndhB_copy2 1533 [131357:132133](+) [132846:133601](+) ndhC 363 [51635:51997](-) ndhD 1503 [110163:111665](-) ndhE 306 [112533:112838](-) ndhF 2220 [104779:106998](-) ndhG 531 [113061:113591](-) ndhH 1182 [116572:117753](-) ndhI 543 [113830:114372](-) ndhJ 480 [50326:50805](-) ndhK 741 [50904:51644](-) petA 963 [61720:62682](+) petB 648 [73499:73504](+) [74329:74970](+) petD 483 [75159:75166](+) [75915:76389](+) petG 114 [65935:66048](+) petL 96 [65660:65755](+) petN 90 [19009:19098](-) psaA 2253 [41101:43353](-) psaB 2205 [38871:41075](-) psaC 246 [111785:112030](-) psaI 111 [59332:59442](+) psaJ 129 [66838:66966](+) psbA 1062 [91:1152](-) psbB 1527 [71066:72592](+) psbC 1422 [9996:11417](+) psbD 1062 [8987:10048](+) psbE 252 [64184:64435](-) psbF 120 [64054:64173](-) psbH 222 [73148:73369](+) psbI 111 [7671:7781](+) psbJ 123 [63667:63789](-) psbK 186 [7087:7272](+) psbL 117 [63915:64031](-) psbM 105 [18168:18272](+) psbN 132 [72913:73044](-) psbT 108 [72757:72864](+) psbZ 189 [12001:12189](+) rbcL 1434 [56653:58086](+) rpl14 372 [79494:79865](-) rpl16 417 [81468:81476](-) [79975:80382](-) rpl2 837 [84552:84956](-) [83460:83891](-) rpl20 360 [68592:68951](-) rpl22 450 [82399:82848](-) rpl23 282 [84963:85244](-) rpl23_copy2 282 [136983:137264](+) rpl2_copy2 837 [137271:137675](+) [138336:138767](+) rpl32 192 [107919:108110](+) rpl33 201 [67414:67614](+) rpl36 114 [78287:78400](-) rpoA 1020 [76601:77620](-) rpoB 3228 [21259:24486](+) rpoC1 2049 [24524:26572](+) rpoC2 4521 [26772:31292](+) rps11 432 [77685:78116](-) rps12 357 [69648:69761](-) [92257:92499](-) rps12_copy2 357 [69648:69761](-) [129728:129970](+) rps14 312 [38403:38714](-) rps16 258 [5567:5606](-) [4503:4720](-) rps18 513 [67856:68368](+) rps19 282 [82918:83199](-) rps19_copy2 282 [139028:139309](+) rps2 711 [31581:32291](+) rps3 720 [81623:82342](-) rps4 606 [46904:47509](-) rps7 471 [91170:91640](-) rps7_copy2 471 [130587:131057](+) rps8 411 [78946:79356](-) ycf3 519 [45814:45945](-) [44854:45081](-) [43970:44128](-) ycf4 558 [59801:60358](+) ycf68 405 [96465:96869](+) ycf68_copy2 405 [125358:125762](-) 39 tRNA genes (29 are unique) Genes Location trnK-UUU [3908:3945](-) [1369:1402](-) trnQ-UUG [6663:6735](-) trnS-GCU [7902:7989](-) trnS-UGA [11569:11656](-) trnG-UCC [12462:12532](+) trnfM-CAU [13007:13080](-) trnG-UCC_copy2 [13892:13914](-) [13171:13218](-) trnT-GGU [15861:15932](+) trnE-UUC [16467:16539](+) trnY-GUA [16601:16684](+) trnD-GUC [17037:17110](+) trnC-GCA [20005:20075](-) trnR-UCU [37955:38026](-) trnfM-CAU_copy2 [38179:38235](-) trnS-GGA [46541:46627](+) trnT-UGU [47812:47884](-) trnL-UAA [48691:48725](+) [49265:49314](+) trnF-GAA [49670:49742](+) trnV-UAC [53556:53594](-) [52923:52959](-) trnM-CAU [53775:53847](+) trnW-CCA [66171:66244](-) trnP-UGG [66375:66449](-) trnH-GUG [83330:83404](+) trnI-CAU [85419:85492](-) trnL-CAA [87980:88060](-) trnV-GAC [94222:94293](+) trnI-GAU [96324:96365](+) [97312:97346](+) trnA-UGC [97412:97449](+) [98261:98295](+) trnR-ACG [102125:102198](+) trnN-GUU [102456:102527](-) trnL-UAG [108828:108907](+) trnN-GUU_copy2 [119700:119771](+) trnR-ACG_copy2 [120029:120102](-) trnA-UGC_copy2 [124778:124815](-) [123932:123966](-) trnI-GAU_copy2 [125862:125903](-) [124881:124915](-) trnV-GAC_copy2 [127934:128005](-) trnL-CAA_copy2 [134167:134247](+) trnI-CAU_copy2 [136735:136808](+) trnH-GUG_copy2 [138823:138897](-) 8 rRNA genes (4 are unique) Genes Location rrn16 [94523:96014](+) rrn23 [98441:101328](+) rrn4.5 [101424:101518](+) rrn5 [101746:101866](+) rrn5_copy2 [120361:120481](-) rrn4.5_copy2 [120709:120803](-) rrn23_copy2 [120899:123786](-) rrn16_copy2 [126213:127704](-) This genome has 21 genes with intron(s). Details are as follows: 20 gene(s) have 1 intron(s) Genes Exons rps12 [69648:69761](-) [92257:92499](-) rps16 [5567:5606](-) [4503:4720](-) atpF [34806:34964](+) [35799:36206](+) rps12_copy2 [69648:69761](-) [129728:129970](+) petB [73499:73504](+) [74329:74970](+) petD [75159:75166](+) [75915:76389](+) rpl16 [81468:81476](-) [79975:80382](-) rpl2 [84552:84956](-) [83460:83891](-) ndhB [90094:90870](-) [88626:89381](-) ndhA [116022:116570](-) [114466:115005](-) ndhB_copy2 [131357:132133](+) [132846:133601](+) rpl2_copy2 [137271:137675](+) [138336:138767](+) trnK-UUU [3908:3945](-) [1369:1402](-) trnG-UCC_copy2 [13892:13914](-) [13171:13218](-) trnL-UAA [48691:48725](+) [49265:49314](+) trnV-UAC [53556:53594](-) [52923:52959](-) trnI-GAU [96324:96365](+) [97312:97346](+) trnA-UGC [97412:97449](+) [98261:98295](+) trnA-UGC_copy2 [124778:124815](-) [123932:123966](-) trnI-GAU_copy2 [125862:125903](-) [124881:124915](-) 1 gene(s) have 2 intron(s) Genes Exons ycf3 [45814:45945](-) [44854:45081](-) [43970:44128](-)